热处理大豆分离蛋白质饲粮对肉鸡胰腺功能的影响

Effects of Heat Treatment of Soy Protein Isolate on Pancreatic Function of Broilers

本试验旨在探讨热处理大豆分离蛋白质饲粮对肉鸡胰腺功能的影响。选用1日龄爱拔益加(AA)肉鸡320只,随机分为4个组,每组8个重复,每个重复10只,分别饲喂4种饲粮:正常大豆分离蛋白质饲粮(对照组),100℃热处理1、4及8 h的大豆分离蛋白质饲粮,试验期为21 d。结果表明:与对照组相比,各热处理大豆分离蛋白质饲粮对14日龄肉鸡胰腺功能相关指标均无显著影响(P>0.05);21日龄,各热处理大豆分离蛋白质饲粮均显著提高了肉鸡胰腺的相对重量(P<0.05),热处理8 h大豆分离蛋白质饲粮使肉鸡胰腺胰蛋白酶和蛋白酶活性显著降低(P<0.05);热处理8 h组肉鸡胰腺丙二醛含量显著升高(P<0.05),氧化型谷胱甘肽含量有升高趋势(P=0.065),但总抗氧化能力(P=0.058)及过氧化氢酶(P=0.064)、谷胱甘肽还原酶(P=0.061)活性有降低的趋势;此外,热处理8 h大豆分离蛋白质饲粮使胰腺中还原型谷胱甘肽/氧化型谷胱甘肽和谷胱甘肽过氧化物酶活性显著下降(P<0.05)。试验结果提示,摄食热处理大豆分离蛋白质饲粮一定时间后会对肉鸡胰腺功能产生负面作用。

This experiment was conducted to investigate the effects of heat treatment soy protein isolate （SPI） on pancreatic function of broilers.A total of 320 one-day-old Arbor Acres broilers were randomly divided into 4 groups with 8 replicates per group and 10 broilers per replicate.Broilers were fed a diet contained normal SPI （control group）,or diets contained SPI heated in an oven at 100 ℃ for 1,4,and 8 h,respectively.The experiment lasted for 21 days.The results showed that compared with the control group,there were no significant differences in pancreatic function parameters of broilers fed diets contained heated SPI at 14 days of age （P＞ 0.05）.The pancreas relative weight of broilers fed diets contained heated SPI was significantly higher than that in control group at 21 days of age （P＜0.05）.The activities of protease and trypsin in pancreas were significantly lower in broilers fed diets contained heated SPI heated for 8 h than those in control group （P＜0.05）.Broilers fed diets contained SPI heated for 8 h exhibited a decrease in total antioxidant capacity （T-AOC） （P =0.058） and catalase （CAT） activity （P=0.064） compared with the control group at 21 days of age.Malondialdehyde （MDA） content of birds fed diets contained SPI heated for 8 h was significantly higher than that in control group at the same time （P＜0.05）.In addition,a tendency toward an increase in glutathione disulphide （GSSG） content （P=0.065） and an decrease in glutathione reductase （GR） activity （P=0.061） in broilers fed diets contained SPI heated for 8 h was observed at 21 days of age.Birds fed diets contained SPI heated for 4 and 8 h showed a significantly decrease in the ratio of reduced glutathione （GSH） to GSSG and glutathione peroxidase （GPx） activity than those in control group at 21 days of age （P＜0.05）.It is concluded that heated SPI ingestion after a specified period can increase the damage of pancreas function.