低分子量岩藻聚糖制备工艺及其抗菌活性的研究

Preparation technology of low-molecular-weight fucoidans and their antibacterial activity

采用超声波辅助过氧化氢的方法降解岩藻聚糖,采用膜分离制备分子量>50 ku和<50 ku 2个组分的低分子量岩藻聚糖组分,并以未降解的岩藻聚糖为对照测定其抗菌活性。通过多糖降解率、硫酸基团保留率和抗菌生物学活性的比较研究证明,超声波辅助降解2 h时的降解效果较好,此时的水解率为30.73%,硫酸基团保留率在24.71%,抑菌圈直径为25 mm。此条件下所制备的低分子量岩藻聚糖具有较好的理化性质及抗菌生物学活性。

In order to prepared low molecular weight fucoidans, the method of ultrasonic-assisted oxidative degradation was used in this work, and hydrogen peroxide was chose as the oxidative agent for its high oxidative capacity. Preparation of the molecular weight by ultrafiltration membrane separation〉50 ku and 〈50 ku two low molecular weight component ingredient fucoidans, and not degraded fucoidans antibacterial activity was measured as a control. Meanwhile, the degradation rate of polysaccharides and sulfate group retention were determined as well as the antibacterial activity on Staphylococcus aureus. The experimental results showed that after two-hours degradation, the rate of hydrolysis of polysaccharides was 30.73%, sulfate group retention rate of low molecular weight fucoidans was 24.71% and the diameter of inhabiting circle for Staphylococcus aureus was 25 mm. Therefore, the method of ultrasonic-assisted oxidative degradation could be effective to degrade fucoidans and the obtained low molecular weight fucoidans exhibited good antibacterial biological activity.