复方中药DF对高血压大鼠血压的影响及机制

Antihypertensive Effect of Compound Traditional Chinese Medicine

目的初步探讨复方中药DF对自发性高血压大鼠(SHR)的降压作用。方法采用水提和醇提法对复方中药DF进行有效成分的提取,用大鼠无创尾动脉法观察口服复方中药DF后对SHR的降压作用,并用相应试剂盒测定血浆甘油三酯、总胆固醇、血管紧张素Ⅱ、内皮素的水平。结果给药3周后水提各剂量组与相应的醇提各剂量组比较有更强的抗高血压的作用(P<0.05)。与不给药对照组比较,水提组能明显降低甘油三酯、总胆固醇、血管紧张素Ⅱ和内皮素水平(P<0.05)。结论复方中药DF水提部分对SHR有明显降压作用,其作用机制可能与降低体内总胆固醇、甘油三酯及舒张血管平滑肌有关。

Objective To investigate the antihypertensive effect and the mechanism of the Chinese medicine compound DF in spontaneous hypertensive rats （SHR）. Methods Effecctive components were extracted by water or alcohol in the Chinese medicine compound DF. Blood pressures of SHR rats were measured indirectly by tail arteries in awaken rats. The level of triglyceride, total cholesterol, the Angiotensin Ⅱ and the endothelin in blood was measured by the corresponding ELISA kits. Results The blood pressure of water extrations were more decreased than that of alcohol extaction in later days after administration. Compared with the model group, the triglyceride, the total cholesterol, the Angiotensin Ⅱ were reduced significantly in the Aqueous extract solution low-dose group of Chinese medicine compound DF. Conclusions The Chinese compound formula DF has a significant antihypertensive effect in SHR. The mechanism may be related to its actions in reduction of triglyceride, total cholesterol, the Angiotensin Ⅱ and the endothelin level in blood.