大花三色堇热激蛋白70(HSP70)基因片段的分离

Cloning of Gene Fragment of HSP70 in Viola×wittrockiana

为了获得三色堇耐热性相关的基因HSP70基因,根据GenBank上登录的拟南芥、陆地棉等HSP70基因序列设计引物,用同源克隆法分离出大花三色堇HAR的HSP70基因片段,并进行测序与比对。结果表明:克隆得到基因片段,长度为882 bp,其序列与已公布的几种植物HSP70的编码区相似率平均为85.1%,上游引物含于基因片段中。初步证明此基因片段为三色堇HSP70基因片段。

In order to obtain the heat resistance gene HSP70 of Violawittrockiana, the primers were designed based on the sequence of the HSP70 gene sequences of Arabidopsis thaliana, Gossypium hirsutum and other plants in GenBank. The HSP70 gene fragment of pansy HAR were cloned by homology cloning and the fragment was sequenced. The length of gene fragment of HAR were 882 bp, the gene sequence shared 85.1% similar rate with the CDS of HSP70, and the forward primer was included in the fragment. The results provided evidence that the gene fragment were the HSPT0 of pansy HAR.