解淀粉芽孢杆菌对山茶灰斑病菌的抑制作用

Bacillus amyloliquefaciens Antimicrobial Effect on Pestalotiopsis guepini (Desm.) Stey

解淀粉芽孢杆菌(Bacillus amyloliquefaciens)及其分泌物对山茶灰斑病病原菌茶褐斑拟盘多毛孢(Pestalotiopsis guepini(Desm.)Stey)具有较好的拮抗作用。采用正交试验法探究了碳源、氮源、pH值、温度、接种量等因子对解淀粉芽孢杆菌产拮抗物质的影响。结果表明:最佳碳、氮源分别为葡萄糖和蛋白胨。最佳优化条件为25℃、蛋白胨0.3 g、葡萄糖0.5 g、pH值6.0、每125 mL接种2 mL孢子悬液于300 mL锥形瓶中震荡培养。基于生长曲线的测定,通过研究解淀粉芽孢杆菌培养时间与代谢产物拮抗活性的关系,得到解淀粉芽孢杆菌对茶褐斑拟盘多毛孢的最佳作用时间为72 h,抑菌值达0.85。

Bacillus amyloliquefaciens and its antagonistic substance can effectively antagonize Pestalotiopsis guepini （Desm.） Stey. By orthogonal design method, we studyied the effects of varied factors on antagonistic substance producing of B. amyloliquefacieus, including carbon source, nitrogen source, cuhural pH, temperature, medium oxygen and inoculated volume. The optimal carbon source and nitrogen source were glucose and peptone, and the optimal fermentation conditions were with 28 ℃ pH of 7, peptone of 0.3 g, and 125 mL medium volume in 300 mL triangle flask bulk with 2 mL inoculating volume. By studying the growth curve and the relationship between culture time and antagonistic activities, we got the bactriostatic effect of B. amyloliquefacieus on P. guepini, and when the optimal reaction time was 72 h, the inhibiting rate was up to 85%.