N-月桂酰肌氨酸钠/丝素蛋白水凝胶的研究

Study on Sodium N-Lauroyl Sarcosine/Silk Fibroin Hydrogel

为实现水凝胶在生物医用材料领域更高的应用价值,丝素蛋白水凝胶需要满足胶凝时间可控并且具有良好细胞相容性。文章采用N-月桂酰肌氨酸钠(SNS)与丝素蛋白(SF)水溶液共混制备水凝胶,讨论了所形成的水凝胶(SNS/SF)胶凝时间与温度、丝素浓度、SNS质量浓度之间的关系。采用傅里叶红外光谱图和X射线衍射曲线表征了其结构,采用扫描电镜观察其内部孔结构,在水凝胶表面接种L929细胞验证其细胞相容性。结果表明:SNS/SF水凝胶的胶凝时间与温度、SNS质量浓度成反比,水凝胶中的丝素蛋白分子形成β-折叠结构。L929成纤维细胞在凝胶上呈对数稳定增长,说明水凝胶具有良好的细胞相容性,具有用于组织修复的潜力。

To achieve higher application value of hydrogel in the field of biomedical materials,silk fibroin hydrogel needs to meet controllable gelation time and good cytocompatibility. Hydrogel was prepared through mixing sodium Nlauroyl sarcosine（ SNS） and silk fibroin（ SF） aqueous solution. The relations between gelation time of hydrogel（ SNS/SF） and the temperature,the concentration of fibroin as well as mass concentration of SNS were discussed.FTIR spectrogram and X-ray diffraction curve were adopted to characterize the structure. SEM was used to observe internal hole structure. The cytocompatibility was verified through inoculating L929 cells on the surface of hydrogel.The results show that： gelation time of SNS /SF hydrogel is inversely proportional to the temperature and mass concentration of SNS; silk fibroin molecules in the hydrogel form β-sheet structure. L929 fibroblasts present logarithmic stable growth on the hydrogel. This indicates hydrogel has favorable cytocompatibility and has the potential to repair tissue.