O-羧甲基壳聚糖复凝止血海绵的体外表征与止血作用

In vitro Characteristics and Hemostatic Effect of O-carboxymethyl Chitosan Multi-hemostatic Sponge

目的研究自制O-羧甲基壳聚糖复凝止血海绵的理化性质,并考察其止血效果。方法采用冷冻干燥技术制备O-羧甲基壳聚糖复凝止血海绵。通过观察外部形态,测定孔隙率、吸水率、密度等指标,表征止血海绵的理化性质;比较O-羧甲基壳聚糖止血海绵和市售吸收性明胶海绵对兔耳动、静脉创伤出血模型的止血效果。结果 O-羧甲基壳聚糖复凝止血海绵呈类白色,疏松多孔,具有一定的韧性,网状结构均匀;孔隙率为67.23%,吸水率为38.77%,密度为0.043 4 g·(cm3)-1;该止血海绵的止血时间和出血量均低于市售吸收性明胶海绵,且无续发性再出血现象。结论该O-羧甲基壳聚糖复凝止血海绵理化性质稳定,止血效果可靠。

Objective To investigate the characteristics in vitro and hemostatic function of O -carboxymethyl chitosan multi-hemostatic sponge. Methods Freeze-drying technology was applied on preparation of O -carboxymethyl chitosan hemostatic sponge. The physicochemical properties of the self-produced multi-hemostatic sponge were observed for its appearance, porosity rate,water absorption,and density. The hemostatic effect was compared between the self-produced hemostatic sponge and commercially available absorbable gelatin sponge in rabbit models with ear venous and arterial injuries. Results The self-produced hemostatic sponge was off-white and full of pores with good tenacity and even net texture. Its porosity ratio, water absorption rate, and density were 67. 23%,38. 77%,and 0. 043 4 g·（cm3）-1,respectively. The bleeding time volume were significantly lower from the self-produced sponge than that from the commercially available gelatin sponge. No secondary re-bleeding was observed. Conclusion The self-produced O -carboxymethyl chitosan multi-hemostatic sponge displays stable physicochemical characteristics and reliable hemostatic effect.