烧伤愈合膏龙脑与异龙脑的含量测定

Determination of Camphol and Isoborneol in Shaoshang Yuhe Gao

目的：建立烧伤愈合膏中龙脑、异龙脑的含量测定方法。方法采用毛细管气相色谱法,以聚乙二醇20000为固定液,涂布浓度为100%,氮气为载气,氢火焰离子化（FID）检测器,用ZB-WAX毛细管柱（30 m×0.25 mm,0.25μm）,程序升温80℃（5 min）→5℃·min-1→180℃（10 min）,分流进样,分流比为101。结果龙脑在0.4875-31.25 ng（r=0.9996,n=6）,异龙脑在0.4875-31.25 ng（r=0.9997,n=6）线性关系良好,平均加样回收率分别为95.95%和96.44%。结论所建立的方法可同时对龙脑、异龙脑的含量进行测定,准确度高,灵敏性好,可用于烧伤愈合膏的质量控制。

Objective To establish a capillary gas chromatography method for determination of camphol and isoborneol in Shaoshang yuhe gao （ burn healing cream） . Methods The capillary gas chromatography was adopted under the following conditions： use PEG-2000 as the stationary liquid,nitrogen as carrier gas,ZB-WAX （30 m×0. 25 mm,0. 25 μm） as the chromatographic column,and the flame ionization detector. The column temperature was programmed at 80 ℃ for 5 min as the initial temperature,then raised to 180 ℃ at the rate of 5℃·min-1 and kept for 10 min. The shunt ratio was 101. Results The liner range for camphol was 0. 487 5-31. 25 μg （ r =0. 999 6）,and the average recovery was 95. 95%（ n =6）. The liner range for isoborneol was 0. 487 5-31. 25 μg（ r =0. 999 7）,and the average recovery was 96. 44%（ n =6）. Conclusion The method is accurate,sensitive,and can be applied to quality control of shaoshang yuhe gao.