Caspase-9在乳腺浸润性导管癌组织中的表达及其临床意义

Expression of Caspase-9 in breast infiltrating ductal carcinoma and its clinical significance

目的:探讨半胱氨酸天门冬氨酸特异的蛋白酶9(Caspase-9)在乳腺浸润性导管癌组织中的表达及其与临床病理因素的关系。方法:采用免疫组织化学SP方法研究38例乳腺浸润性导管癌(breast infiltrating ductal carcinoma,IDC)、32例乳腺纤维腺瘤及其肿物旁的正常乳腺组织中Caspase-9的表达水平,观察评估Caspase-9的阳性表达与患者年龄、肿瘤大小、淋巴结转移等临床病理因素的相关性,并探讨与乳腺浸润性导管癌的临床分期、组织学分级的关系。结果:乳腺浸润性导管癌、乳腺纤维腺瘤组织中的Caspase-9的阳性表达均低于正常乳腺组织(P<0.05),且乳腺浸润性导管癌明显低度表达(P<0.01)。其表达水平与患者年龄、肿瘤大小、淋巴结转移等不相关(P>0.05),与临床分期、组织学分级明显负相关(P<0.01)。结论:Caspase-9低表达与乳腺浸润性导管癌的恶化显著相关,该指标有助于乳腺浸润性导管癌的预后判断,其可能成为潜在乳腺肿瘤标志物,并成为治疗的靶点。

Objective： To investigate the expression of Caspase-9 protein in breast infiltrating ductal carcinoma（ IDC） and its clinical significance. Methods： The expression of Caspase-9 was detected by streptavidin-perosidase（ SP） with tissues of thirty-eight cases of IDC and thirty-two cases of fibroadenoma,including normal tissues adjacent to tumor. Positive expression of Caspase-9 relation with clinicopathological factors such as age,tumor size,lymph node metastasis were observed. Furthermore,clinical stage and histopathologic grade were explored in IDC. Results： The expression of Caspase-9in IDC tissues and fibroadenoma tissues were lower than that in adjacent normal tissues（ P〈0. 05）. Meanwhile,the IDC tissues was significantly down-regulated（ P〈0. 01）. The expression of Caspase-9 was independent of clinicopathological parameters,such as age,tumor size,lymph node metastasis（ P〉0. 05）. However,It had obvious negative correlation with clinical stage and histopathologic grade（ P〈0. 01）. Conclusion： The lower expression of Caspase-9 closely correlates to the progress of breast infiltrating ductal carcinoma. The index greatly contributes to judgment of prognosis of breast infiltrating ductal carcinoma. Indicating that Caspase-9 might be used as a tumor biomarker and a potential therapeutic target for inhibiting the invasive of breast infiltrating ductal carcinoma.