摘要
为鉴别我国牛种布鲁氏菌疫苗株A19与野生菌株,运用生物信息学方法结合基因测序,对疫苗株A19基因组单核苷酸多态性(SNP)位点分析筛选,选取其中部分SNP位点,通过与布鲁氏菌常见种、生物型标准参考菌株和疫苗株基因组SNP位置核苷酸测序比较,验证SNP位点的A19特异性。结果表明,共筛选获得A19基因组29个SNP位点,验证ClpX G825-C825、LysR A605-C605、Omp2b G503-A503这3个SNP位点为A19(或S19)特异,揭示了A19基因组SNP位点分布情况,为疫苗株A19与野生菌株鉴别提供了分子依据。
To distinguish Brucella abortus vaccine strain A19 from field strains, the single nucleotide polymorphism （SNP） signatures for B. abortus A19 were initially analyzed using bioinformatics method and gene sequencing. Then, the specificity of several SNPs was verified, by comparing the nucleotide sequences of these SNPs with common species and biovars of Brucella and three Brucella vaccines. The results showed 29 SNPs were successfully screened from genome of B. abortus A19. Furthermore, ClpX G825-C825,LysR A605-C605and Omp2b G503-A503were confirmed to be specific to B. abortus A19 （or B. abortus S19）. Our study systematically revealed the SNP distribution of B. abortus A19, which provided a molecular basis for differentiating B. abortus A19 from field strains.
出处
《中国兽药杂志》
2014年第7期1-5,共5页
Chinese Journal of Veterinary Drug
基金
青岛市科技成果转化引导计划(青年专项)(14-2-4-79-jch)
