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Sp2对宫颈癌HeLa细胞增殖的影响及Cyclin D1的表达变化 被引量：6

Effects of Sp2 on the proliferation of cervical cancer Hela cells and expression of Cyclin D1
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摘要 目的研究Sp2对宫颈癌Hela细胞增殖的影响及其分子机制。方法应用Real-time PCR和Western blot分析宫颈癌组织中Sp2表达变化及其Sp2siRNA对Sp2和Cyclin D1mRNA及蛋白表达的影响;MTT比色法分析Sp2对宫颈癌Hela细胞增殖活力的影响;采用流式细胞仪分析对细胞周期和细胞凋亡的影响。结果 Sp2在宫颈癌组织中表达显著上调;Sp2siRNA抑制宫颈癌Hela细胞增殖;Sp2siRNA组与对照组相比,S期与G2/M期细胞数量显著减少(P<0.01),G1/G0期细胞数量显著增加(P<0.01),而且Sp2与Cyclin D1在mRNA和蛋白水平的表达均显著下调(P<0.01)。结论 Sp2通过上调Cyclin D1的表达促进宫颈癌Hela细胞增殖。 Objective To investigate the effects of transcription factor （Sp2） on the proliferation of cervical cancer Hela cells and the molecular mechanisms.Methods The expression of Sp2 in cervical cancer tissues and effects of Sp2 siRNA on the expressions of Sp2 and Cyclin D1 RNA and protein were analyzed by Real-time PCR and Western Blot,respectively.MTT assay was used to analyze the effects of Sp2 on the proliferation of cervical cancer Hela cells.The effects of Sp2 on cell cycle and apoptosis of cervical cancer Hela cells were analyzed with flow cytometer.Results Sp2 expression level was higher in human cervical cancer tissues than in normal cervical tissues.Sp2 siRNA inhibited the proliferation of cervical cancer Hela cells.The number of S and G2/M phase cells significantly decreased in Sp2 siRNA group compared with the control group （P＜0.01）.Meanwhile,the number of G1/G0 phase cells increased remarkably （P＜0.01）.It was found that Sp2 and Cyclin D1 expressions at the mRNA and protein levels downregulated significantly in Sp2 siRNA group compared with the control group （P＜0.01）.Conclusion Sp2 promotes the proliferation of cervical cancer Hela cells through up-regulating Cyclin D1 expression.
作者 袁渊 郑鹏生 周敏 姚安梅 王静
机构地区 西安交通大学医学院 陕西省肿瘤医院妇瘤科
出处 《西安交通大学学报（医学版）》 CAS CSCD 北大核心 2014年第4期481-485,共5页 Journal of Xi’an Jiaotong University（Medical Sciences）
关键词 SP2 宫颈癌 增殖 细胞周期 CYCLIN D1 SIRNA 凋亡 transcription factor 2 （Sp2） cervical cancer proliferation cell cycle Cyclin D1 siRNA apoptosis
分类号 R737.33 [医药卫生—肿瘤]
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参考文献20

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西安交通大学学报（医学版）
2014年 第4期

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