纤维蛋白原β链启动子区域-455单核甘酸多态性与胃癌的相关性

Study on the Relevance Between Fibrinogen β Chain Promoter Regions-455 Single Nucleotide Polymorphism and Gastric Cancer

目的：探讨纤维蛋白原（Fg）基因β链启动子区域-455 G/A与胃癌发生的相关性.方法：100例胃癌患者为观察组,与观察组年龄匹配的健康体检者100例为对照组,采集两组受检者外周静脉血,用氯仿-异丙醇法提取DNA,应用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）和HindⅢ限制性内切酶、分别检测Fgβ-455G/A位点基因型.结果：（1）对照组Fgβ-455位点等位基因G和A的频率分别为0.745、0.255,基因型分布符合Hardy-Weinberg平衡定律;（2）胃癌组Fgβ-455位点GG、GA、AA基因型频率分别为69％、29％和2％,对照组分别为57％、35％和8％,两组比较P＞0.05;Fgβ-455-G、A等位基因频率在胃癌组与对照组分别为0.835、0.745和0.165、0.255,两组比较P＜0.05;（3）突变纯合子AA型与胃癌的发病有关联（OR=0.842,95％ CI=0.362～9.323,P=0.034）,显著降低胃癌的发病风险.结论：Fgβ-455G/A位点基因多态性可能与胃癌发病有关,A等位基因可能对胃癌发病是保护性基因.

Objective： To investigate the relevance between Fibrinogen gene beta chain promoter re- gions-455 gene and gastric cancer. Methods： 100 gastric cancer patients were selected as observation group, 100 same age healthy controls as control group. Collecting peripheral venous blood of both groups, DNA was extracted by chloroform-isopropyl alcohol method. Then, adopting PCR-RFLP and Hind II[ restriction enzymes to detect gene in BB-455-g/A polymorphism loci. Results： （1）Fgβ-455 loci alleles G and A frequency were 0. 745 and O. 255 in control group, genotype distribution was con- sistent with Hardy-Weinberg equilibrium principle ; （ 2 ） Fg13-455-g gene GG, GA, AA, respectively were 69%, 29% and2% in observation group, and 57% ,35% and 8% of that of control group, com- parison differences P 〉0.05 ; （3） Fgβ-455-G, A allele were 0. 835 and 0. 745, 0. 165 and 0. 255 in both groups, comparison differences P 〈 0.05 ; （3） Homozygous mutations of AA type was relevant with the onset of gastric cancer （ OR = 0. 842 ;95 % CI was 0. 362 ~ 9. 323 ; P = 0. 034 ）, which is signifi- cant in reducing the risks of gastric cancer. Conclusions： Fgβ-455 G/A polymorphism of chain pro- moter regions has potential relations with the onset of gastric cancer, A allele is possible to serve as pro- tective genes for onset of gastric cancer.