紫外可见分光光度法测定深裂竹根七多糖的含量

Determination of Polysaccharide Content in Disporopsis pernyi by UV Spectrophotometry

目的：建立深裂竹根七多糖含量测定的方法.方法：选用D-葡萄糖为对照品,4％的蒽酮浓硫酸为显色剂,用分光光度法在626 nm处进行深裂竹根七多糖含量测定.结果：D-葡萄糖回归方程为y=9.101 9x＋0.0441,在0.010 8～0.097 2 g/L浓度范围内线性关系良好（r2 =0.999 3）,深裂竹根七中多糖的平均回收率为97.65％ （RSD =2.360％,n=6）,重现性好（RSD =2.2％,n=5）,精密度高（RSD=0.02％,n=5）.结论：紫外分光光度法可简便、灵敏和准确检测深裂竹根七多糖的含量,可以作为该药材的质量控制方法.

Objective： To establish a method for the determination of polysaccharide in Disporopsis pernyi. Methods： Polysaccharide content in Disporopsis pernyi was determined with UV-visible spectro- photomety at 626 nm used D-glucose as control sample, 4% of anthrone-sulfuric acid as chromogenic agent. Results： The regression equation of D-glucose was y -- 9. 101 9x ± 0.044 1,. the linear rang of the method was 0.010 8 ±0.097 2 g/L （r2 =0. 999 3）, The average recovery was 97.65% （ RSD = 2. 360%, n = 6 ）, with good repeatability （ RSD = 2.2%, n = 5 ） and high precision （ RSD = 0. 02% ,n =5）. Conclusions： This method is simple, sensitive and accurate and can be used to de- termine the polysaccharide content and control the quality of Disporopsis pernyi.