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动物病毒核酸检测质控品和标准物质研究进展 被引量：8

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摘要 近年来，以核酸扩增为基础的分子检测技术，以其快速、敏感、特异、定量准确，而且对实验室生物安全环境要求低等特点，在动物疫病病原的检测中得到了广泛的应用，逐渐成为动物疫病检测的主流技术。
作者 乔彩霞 张鹤晓 高志强 蒲静 汪琳 刘环
机构地区 北京出入境检验检疫局
出处 《中国预防兽医学报》 CAS CSCD 北大核心 2014年第7期582-584,共3页 Chinese Journal of Preventive Veterinary Medicine
基金 国家质检总局科技计划项目(2012IK002)
关键词 病毒核酸检测 动物疫病 标准物质 质控 分子检测技术 核酸扩增 安全环境 疫病检测
分类号 S852.65 [农业科学—基础兽医学]
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参考文献18

  • 1Bel6k S, Thor6n P, LeBlanc N, et al. Advances in viral disease diagnostic and molecular epidemiological technologies [J]. Expert Rev Mol Diagn, 2009, 9(4): 367-381.
  • 2Fronhoffs S, Totzke G, Stier S, et al. A method for the rapid construction of cRNA standard curves in quantitative real-time reverse transcription polymerase chain reaction [J]. Mol Cell Probes, 2002, 16: 99-110.
  • 3Madej R M, Davis J, Holden M J, et al. International standards and reference materials for quantitative molecular infectious dis- ease testing [J]. J Mol Diagn, 2010, 12(2): 133-143.
  • 4李金明.RNA病毒扩增检测的质控品和标准品研究进展[J].中华检验医学杂志,2004,27(12):873-874. 被引量：19
  • 5Neisters H G M. Clinical virology in real time [J]. J Clin Virol, 2002, 25: 3-12.
  • 6Hersberger M, Charly N, Scholer A, et al. Influence of practi- cable virus inactivation procedures on tests for frequently mea- sured analytes in plasma [J]. Clin Chem, 2004, 50(5): 944-946.
  • 7A1-Soud W A, Radstrom E. Purification and characterization of PCR-inhibitory components in blood cells [J]. J Clin Microbiol, 2001, 39(2): 485-493.
  • 8Dhanasekaran S, Doherty T M, Kenneth J, et al. Comparison of different standards for real-time PCR-based absolute quantifica- tion [J]. J Immunol Methods, 2010, 354: 34-39.
  • 9Matthews A, Chung M, Matyas R J. Persistent DNA contamina- tion in competitive RT-PCR using eRNA intemal standards: identity, quantity, and control [J]. Biotechniques, 2002, 32 (6): 1412-1414.
  • 10Gardiol V D, Miguel A. Strategic approach to produce low-cost, efficient, and stable competitive internal controls for detection of RNA viruses by use of reverse transcription-PCR_Gabriela V [J]. J Clin Microbiol, 2007, 12: 3555-3563.

二级参考文献14

  • 1Fronhoffs S, Totzke G, Stier S, et al. A method for the rapid construction of cRNA standard curves in quantitative real-time reverse tanscription polymerase chain reaction. Mol Cell Probes, 2002,16:99-110.
  • 2Alms WJ, Braun-Elwert L, James SP, et al. Simultaneous quantitation of cytokine mRNAs by reverse transcription-polymerase chain reaction using multiple internal standard cRNAs. Diagn Mol Pathol, 1996,5: 88-97.
  • 3Kim K, Park J, Chung Y, et al. Use of internal standard RNA molecules for the RT-PCR amplification of the faeces-borne RNA viruses. J Virol Methods, 2002,104:107-115.
  • 4Hazari S, Acharya SK, Panda SK. Development and evaluation of a quantitative competitive reverse transcription polymerase chain reaction (RT-PCR) for hepatitis C virus RNA in serum using transcribed thio-RNA as internal control. J Virol Methods, 2004,116:
  • 5Matthews JL, Chung M, Matyas RJ. Persistent DNA contamination in competitive RT-PCR using cRNA internal standards: identity, quantity, and control. Biotechniques, 2002,32:1412-1414, 1416-1417.
  • 6Pickett GG, Peabody DS. Encapsidation of heterologous RNAs by bacteriophage MS2 coat protein. Nucleic Acids Res, 1993,21:4621-4626.
  • 7Stockley PG, Stonehouse NJ, Murray JB, et al. Probing sequence-specific RNA recognition by the bacteriophage MS2 coat protein. Nucleic Acids Res, 1995, 23: 2512-2518.
  • 8Cartwright CP. Synthetic Viral Particles Promise to Be Valuable in the Standardization of Molecular Diagnostic Assays for Hepatitis C Virus. Clin Chem, 1999,45: 2057-2059.
  • 9Cindy R, Peach W, Winkler M, et al. Ribonuclease-resisting RNA controls (armored RNA) for reverse transcription-PCR,branched DNA, and genotyping assays for hepatitis C virus. Clin Chem, 1999,45:2079-2085.
  • 10Pasloske BL, Walkerpeach CR, Obermoeller RD, et al. Armored RNA technology for production of ribonuclease-resistant viral RNA controls and standards.J Clin Microbiol, 1998,36:3590-3594.

共引文献18

同被引文献93

引证文献8

二级引证文献11

中国预防兽医学报
2014年 第7期

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