结直肠癌中Runx3和C-myc基因的表达

Expression of Runx3 and C-myc in human colorectal cancer

目的探讨Runx3和C-myc基因在结直肠癌组织中的表达及其与临床病理参数的关系。方法采用实时荧光定量PCR技术检测38例结直肠癌病人的癌组织及相应癌旁正常组织(距癌缘>5 cm)中Runx3 mRNA和C-myc mRNA的表达水平;Western-blot免疫印迹法检测63例结直肠癌病人的癌组织及相应癌旁正常组织中Runx3蛋白和C-myc蛋白的表达水平。将实验数据按照临床病理特点进行分层分析,采用统计软件SPSS 13.0进行统计,了解Runx3、C-myc的表达与结直肠癌患者主要临床病理参数之间的关联性。结果 mRNA水平与蛋白水平提示:Runx3在结直肠癌组织中的表达下调,C-myc在结直肠癌组织中的表达上调,二者的蛋白表达呈负相关(Protein levels,r=-0.398,P=0.001)。且其各自的表达在不同Dukes分期、肿瘤浸润深度、淋巴结转移状态及病理分化类型差异均具有统计学意义(P<0.05),临床分期越晚、病理分化越低,Runx3表达下调、C-myc表达上调越明显。结论 Runx3和C-myc基因表达异常,参与了结直肠癌的发生、发展,并与病员临床病理参数密切相关。

Objective To investigate Runx3 and C-myc expressions in colorectal cancer and their relationship with the clinicopathological parameters. Methods Real-time quantitative PCR was used to detect Runx3 and C-myc mRNA expressions in 38 colorectal cancer tissues and matched adjacent tissues, and Runx3 and C-myc expressions was detected by Western blotting in 63 pairs of colorectal cancer and adjacent tissues. The results were stratified according to the clinicopathological characteristics to examine the relationship of Runx3 and C-myc expressions with the clinicopathological factors in the patients. Results Runx3 expression was down-regulated and C-myc expression up-regulated at both mRNA and protein levels in colorectal cancer tissues compared with the normal tissues, and their protein expressions exhibited an inverse correlation （r=-0.398, P=0.001）. Runx3 and C-myc expressions differed significantly between tumors with different Dukes stages, depths of tumor invasion, lymph node statuses, or histological differentiation （P〈0.05）;Runx3 down-regulation and C-myc up-regulation were more obvious in tumors in advanced Dukes stage and in poorly differentiated tumors. Conclusion Abnormal expressions in Runx3 and C-myc may contribute to the occurrence and development of colorectal cancer and are closed correlated with the patient＇s clinicopathological parameters.