STZ诱导的2型糖尿病大鼠心肌组织TRPC7的表达上调

Expression of cardiac TRPC7 channels increases in streptozotocin-induced diabetic rats

目的:观察2型糖尿病(T2DM)大鼠心肌组织损伤过程中,TRPC7的表达变化。方法:以高脂饲料喂养并辅以腹腔注射小剂量链脲佐菌素(Streptozotocin,STZ)来建立T2DM大鼠模型,采用蛋白印迹及免疫组化染色方法检测TRPC7在T2DM大鼠心肌组织的表达变化。结果:1T2DM大鼠造模成功且T2DM大鼠心肌组织发生损伤性变化:与正常组大鼠相比,T2DM组大鼠血糖明显升高且体重显著降低(P<0.05);T2DM组大鼠心脏体积与质量显著减小(P<0.05);T2DM组大鼠心脏质量指数(heart weight index,HWI)明显高于正常组(P<0.05);2在T2DM大鼠心肌损伤过程中TRPC7表达上调:TRPC7在正常及T2DM大鼠心肌组织中均有表达,但与正常大鼠相比,T2DM大鼠心肌组织TRPC7蛋白的表达显著增高(P<0.05)。结论:首次证实在损伤的T2DM大鼠心肌组织中,TRPC7的表达显著上调。

AIM： To investigate the change of cardiac TRPC7 expression in streptozotocin （STZ）-induced type 2 diabetic rats. METHODS： STZ-induced type 2 diabetic rat models were established and the expression of cardiac TRPC7 channels was evaluated by immunofluoreseence and Western blot. RESULTS ： 1 ） The impaired heart was found in STZ-induced T2DM rats. 2） The expression of cardiac TRPC7 signifi- cantly increased in STZ-induced T2DM rats as compared with that in control rats. CONCLUSION： The present work makes a novel finding that the expression of cardiac TRPC7 channels significantly increased in the impaired hearts of STZ-induced type 2 diabetic rats.