缺血后大鼠电刺激嗅觉诱发电位研究

Electrically stimulated olfactory evoked potential in ischemical rats

目的观察大鼠缺血损伤后不同时间电刺激嗅觉诱发电位(olfactory evoked potentials,OEP)的变化。方法对照组A组21只,双侧颈总动脉结扎并按照术后1h、2 h、4 h和1天分为B组17只、C组10只、D组6只、E组6只的SD大鼠进行电刺激OEP检测。结果①A组中有18只(85.7%)大鼠可记录到一组由"负-正-负"三相波组成的诱电位波形图,3只(14.3%)为"负-正"两相波组成。N1、P1、N2潜伏期分别为(13.91±1.40)、(24.09±3.19)、(45.32±5.35)ms;N1、P1、N2振幅分别为(17.42±7.71)、(28.57±11.70)、(13.07±5.82)μV。②B组电刺激OEP的N1、P1潜伏期显著延长,E组N2潜伏期显著延长;E组N1振幅显著增大。结论双侧颈总动脉结扎可以导致电刺激OEP的变化,可能造成或加重嗅觉障碍。

OBJECTIVE To investigate the characteristics of olfactory evoked potential at different time in bilateral common carotid artery ligation rats. METHODS The control group A（21 rats） and bilateral common carotid artery ligation group B（17 rats）, group C（10 rats）, group D（6 rats）, group E（6） which according to the postoperative time（1 hours, 2 hours, 4 hours and 1 days）, the electrically stimulated olfactory evoked potential of them were detected. RESULTS 1. 18 rats in group A（21 rats, 85.7%） can be recorded a group of ＇negative–positive-negative＇ three-phase wave; 3 rats（14.3%） can be recorded a group of ＇negativepositive＇ two-phase wave. The latency of N1, P1, N2 were（13.91±1.40）,（24.09±3.19）,（45.32±5.35） ms; the amplitude of N1, P1, N2 were（17.42±7.71）,（28.57±11.70）,（13.07±5.82） μV. 2. The latency of N1, P1 wave in group B were significantly prolonged, the latency of N2 was prolonged in group E; the amplitude of N1 significantly increased in group E. CONCLUSION Bilateral common carotid artery ligation can result in changes of olfactory evoked potential electrical stimulation in rats, and may cause the olfactory dysfunction.