KLF4、KLF6在人膀胱移行上皮癌组织中的表达及临床意义

Expression and clinical significance of KLF4 and KLF6 in human bladder transitional cell carcinoma

目的:观察KLF4、KLF6在人膀胱移行上皮癌组织中的表达,并探讨其对膀胱癌发生、发展的影响。方法:采用实时定量荧光PCR技术检测49例膀胱癌手术切除标本癌组织与25例正常膀胱上皮组织内KLF4、KLF6mRNA的表达,并分析其与患者临床病理特征的关系,采用SPSS 19.0软件进行统计学分析。结果:实时定量荧光PCR结果显示KLF4在膀胱移行上皮癌组织中的相对含量为(0.218±0.046),在膀胱正常上皮组织中的相对含量为(0.311±0.056),二者之间差异有统计学意义(P<0.05)。KLF6在膀胱移行上皮癌组织中的相对含量为(0.198±0.038),在膀胱正常上皮组织中的相对含量为(0.285±0.043),差异有统计学意义(P<0.05)。膀胱移行上皮癌组织中,KLF4与肿瘤的组织学分级、TNM分期及有无淋巴结转移有关(P<0.05),与患者的性别、年龄无关(P>0.05);而KLF6表达与肿瘤的TNM分期有关(P<0.05),与肿瘤组织学分级、淋巴结转移及患者的年龄性别均无关(P>0.05)。结论:KLF4和KLF6表达降低可能与膀胱移行上皮癌的发生和浸润密切相关,通过干预KLF4和KLF6的活性可能成为治疗膀胱移行上皮癌的新靶点。

Objective：To observe the expression and significance of KLF4and KLF6in human bladder transitional cell carcinoma（BTCC）and to analyze the relationship between these two factors and formation and development of BTCC.Method：Real-time fluorescent quantitative PCR（qRT-PCR）was used to detect the expression of KLF4and KLF6mRNA in 49cases of BTCC tissues and 25cases of normal bladder tissues.The relationship between the expression of these two factors and the clinical and pathological features of BTCC patients was analyzed and SPSS 19.0software was used for statistical analysis.Result：The qRT-PCR showed that the relative content of KLF4mRNA in BTCC tissues and normal bladder tissues were（0.218±0.046）and（0.311±0.056）,respectively.The difference between them was statistically significant（P〈0.05）.The relative content of KLF6mRNA in BTCC tissues and normal bladder tissues were（0.198±0.038）and（0.285±0.043）,respectively.The difference between them was statistically significant（P〈0.05）.The expression of KLF4in BTCC was related to histological grade,TNM staging and lymph node metastasis（P〈0.05）,and have little relationship with sex and age（P〉 0.05）.The expression of KLF6in BTCC was related to TNM staging（P〈0.05）,and independent of histological grade,lymph node metastasis,sex and age（P〉 0.05）.Conclusion：The decreased expression of KLF4and KLF6may be closely associated with the carcinogenesis and invasion of BTCC.Intervening activity of KLF4and KLF6may become a new gene target for the treatment of BTCC.