乳腺癌中BAG-1 EGFR和PARP-1的表达及其临床意义

BAG-1,EGFR,and PARP-1 expressions in breast cancer and their clinical significance

目的:观察乳腺癌组织芯片中BAG-1、EGFR和PARP-1的表达及其与临床病理指标的相关性,并探讨其临床意义。方法:采用免疫组织化学法对159例组织芯片中乳腺癌和癌旁组织点行BAG-l、EGFR及PARP-1蛋白检测,同时分析其与临床病理指标的相关性。结果:乳腺癌组织中BAG-1、EGFR、PARP-1蛋白表达水平显著高于癌旁组织(P<0.05)。BAG-1蛋白表达水平与年龄、肿瘤部位、淋巴结转移数目、临床分期均无明显相关性,但与肿瘤大小、组织学分级、ER、PR和HER-2表达以及分子分型有关(P<0.05);EGFR蛋白表达水平与肿瘤大小、临床分期以及分子分型有明显相关性(P<0.05);PARP-1与组织学分级、淋巴结转移数目、ER表达以及分子分型有关(P<0.05)。119例病例中BAG-1蛋白表达与EGFR、PARP-1蛋白表达无明显相关性,但在三阴性乳腺癌(TNBC)中BAG-1蛋白表达与PARP-1蛋白表达呈正相关(P<0.05)。单因素分析结果显示,BAG-1、PARP-1蛋白表达和分子分型是影响乳腺癌患者预后的因素。多因素分析结果显示,BAG-1、PARP-1蛋白表达是影响乳腺癌患者预后的独立危险因素。结论:乳腺癌中BAG-1、EGFR和PARP-1蛋白高表达提示其可能参与乳腺癌的发生发展,BAG-1、EGFR和PARP-1高表达可作为乳腺癌诊治和预后的生物指标。

Objective： To observe BAG-1, EGFR, and PARP-1 expressions in invasive breast cancer and its correlation with clinical pathological indicators, as well as to evaluate their clinical significance. Methods： The BAG-1, EGFR, and PARP-1 expressions in a tissue microarray of invasive breast cancer and peritumoral tissues were detected through immunohistochemical staining. The clinical and pathological significance of BAG-1, EGFR, and PARP-1 were evaluated. Results： The BAG-1, EGFR, and PARP-1 expression levels are higher in invasive breast cancer tissues than in peritumoral tissues （P〈0.05）. BAG-1 expression in invasive cancer tissues is not related to age, tumor site, lymph node metastases, and clinical TNM staging of patients, but is related to size, grade, ER, PR, and HER-2 expressions and molecular subtype （P〈0.05）. EGFR expression is related to size, clinical TNM staging, and molecular subtype （P〈0.05）. PARP-1 expression is related to grade, lymph node metastases, ER, and molecular subtype （P〈0.05）. BAG-1 expression is not significantly correlated with EGFR and PARP-1 in all cases, but BAG-1 and PARP-1 expressions are positively correlated in triple-negative breast cancer tissues （P〈0.05）. Results of the univariate analysis revealed that the BAG-1 and PAP, P-1 expressions and the molecular subtypes are associated with the prognoses of breast cancer patients. Multivariate analysis revealed that BAG-I and PARP-1 expressions are factors that are independent of the prognosis. Conclusion： BAG-1, EGFR, and PARP-1 overexpressions in human breast tissues suggest that BAG-1, EGFR, and PARP-1 are related to breast cancer development. BAG-I, EGFR, and PARP-1 are potential biomarkers of breast cancer diagnosis and prognosis.