顺铂对结直肠癌细胞增殖及侵袭能力的影响

Effects of Cisplatin on Proliferation and Invasion of Colorectal Cancer Cells

目的探讨顺铂对人结直肠癌细胞SW480增殖及侵袭力的影响。方法以SW480细胞为研究对象,设定未经处理的SW480细胞为对照组,给予不同浓度顺铂进行不同时间干预,应用MTT法、Transwell侵袭小室、定磷法检测SW480细胞的增殖、侵袭力及Na+-K+-ATP酶活性。结果生理浓度顺铂(70μmol/L)在48 h即可抑制SW480细胞的增殖,与72和96 h相比抑制率差异无统计学意义;70μmol/L顺铂处理48 h时,即可降低穿越Matrigel膜基质的细胞数;分别采用17.5、35、70和140μmol/L4个梯度浓度的顺铂作用于SW480细胞48 h后,35、70和140μmol/L组细胞中Na+-K+-ATP酶活性均显著升高,且顺铂浓度达70μmol/L时Na+-K+-ATP酶即可达到较高活性。结论 Na+-K+-ATP酶活性的降低可能导致对结直肠癌细胞增殖和侵袭能力调节作用减弱,可能与结直肠癌细胞SW480耐顺铂有关。

Objective To investigate the effect of cisplatin on proliferation and invasion of colorectal cancer cell line SW480. Methods SW480 cells were used as the research object, and untreated SW480 cells were used as the control group. Different concentrations of cisplatin was given at different times to intervene. The MTT, Transwell chamber and phos-phate determination methods were used to detect proliferation, invasion and Na＋-K＋-ATPase activity expression level in SW480 cells. Results The physiological concentration of cisplatin （70 μmol/L） inhibited the proliferation of SW480 cells at 48 h. There was no significant difference in the inhibition rate at 48 h compared with 72 h and 96 h. Treatment with 70 μmol/L cisplatin for 48 h reduced the number of cells through Matrigel membrane matrix. The Na＋-K＋-ATPase activity was significantly increased in 35, 70 and 140 μmol/L cells after treatment with 17.5, 35, 70 and 140 μmol/L of cisplatin in SW480 cells for 48 h, and Na＋-K＋-ATPase reached the highest level at 70 μmol/L of cisplatin. Conclusion The decreased activity of Na＋-K＋-ATPase may lead to the attenuation in proliferation and invasion of colorectal cancer cells, which may be associated with cisplatin resistance in colorectal cancer cell SW480.