TreadScan步态分析系统在评价大鼠脊髓挫伤模型中的应用

Application of TreadScan Gait Analysis System in the Evaluation of Rat Spinal Cord Contusion Model

目的:探讨TreadScan步态分析系统在评价大鼠脊髓挫伤(spinal cord injury,SCI)模型中的应用价值。方法:采用连有PinPoint?精密接触传感器的BenchmarkTM立体定位颅脑撞击器制备脊髓挫伤大鼠模型,利用Image J图像分析软件测定伤后30 min损伤中心及其邻近区域的初始损伤体积,应用TreadScan步态分析系统描述术前及伤后7、14、21、28 d大鼠的行为学特征,采用Harris氏HE染色结合Luxol固蓝染色技术观察伤后1、7、14、28 d损伤组织的形态学改变。结果:模型组脊髓初始损伤体积为(4.17±0.15)mm3(n=5),范围在4.02至4.32 mm3之间,呈近似正态分布。与术前比较,模型组术后各项行为学参数差异均具有显著性(P<0.05);与假手术组比较,伤后模型组脚印面积、步长、瞬间跑速、正常步序比均下降显著(P<0.05),而跨步时间、摆动时间、后脚脚间距、最小纵向偏差均显著上升(P<0.05)。伤后1 d除损伤中心外,邻近区域组织形态基本完好,但是随时间延长,模型组组织溃变和髓鞘脱失的严重程度显著高于假手术组,且有向周边蔓延趋势。结论:采用连有PinPoint?精密接触传感器的BenchmarkTM立体定位颅脑撞击器制备的脊髓挫伤大鼠模型具有良好的重复性,应用TreadScan步态分析系统能够客观准确的评价大鼠SCI后的运动功能。

Objective：To assess the utility of TreadScan gait analysis system in evaluating rat spinal cord contusion model. Methods：Rat spinal cord contusion model was established by a BenchmarkTM stereotaxic cortical impactor which was connected to PinPointTM precision contact sensor. Image J image analysis software was used to measure the initial lesion volume of injured epicenter and adjacent area at posttraumatic 30 min. TreadScan gait analysis system was applied to describe the behavioral characteristics of the rats at preoperative and posttraumatic 7, 14, 21, 28 d. Morphological changes were inspected by using the Harris’s HE staining in combination with Luxol fast blue staining technique at posttraumatic 1, 7, 14, 28 d. Results： The initial＆nbsp;lesion volume of spinal cord in model group was （4.17±0.15） mm3 （x±s, n=5） with a range of 4.02 to 4.32 mm3 and fitted to the normal distribution. As compared to the preoperative, there were significant differences （P〈0.05） in each behavior parameters of the postoperative in model group. As compared to the sham group, the Average Print Area, Stride Length, Inst Run Speed and Regularity Index were decreased significantly in model group （P〈0.05）. However, the Stride Time, Swing Time, Rear Track Width, Minimum Longitudinal Deviation were significantly increased （P〈0.05）. In addition to the injured epicenter, the morphology of adjacent region tissue was basically intact at posttraumatic 1 d. However, overtime the severity of tissue degeneration and demyelination in model group were significantly higher than those in sham group, with the trend of spreading to the surrounding areas. Conclusion： The rat spinal cord contusion model prepared by a BenchmarkTM stereotaxic cortical impactor has a good replicability. TreadScan gait analysis system was particularly objective and accurate in evaluating the behavioral characteristics after SCI in rats.