摘要
目的采用大孔树脂柱色谱分离甘草总黄酮。方法通过吸附率和解吸率的测定,在9种不同型号的大孔树脂中,选择最适于分离甘草总黄酮的大孔树脂,并优化了洗脱液的浓度。结果 AB-8型大孔树脂的吸附和解吸性能均较好,对甘草总黄酮的吸附率为68.0%,解吸率为83.5%,适用于甘草黄酮的分离。结论 AB-8型大孔树脂是一种较好的分离甘草总黄酮的树脂材料。
Objective To separate total flavonoids from licorice by macroporous resin column chromatography.Methods The screening of the resin best suited for the separation of total flavonoids from licorice among 9 types of macroporous resins was accomplished by determination of adsorption rate and desorption rate,and the concentration of the eluant was optimized.Results AB-8 resin showed best separation performance,with absorption rate of 68.0 ％,and desorption rate of 83.5 ％.Conclusion AB-8 resin was suitable for the separation of licorice flavonoids.
出处
《食品与药品》
CAS
2013年第6期427-428,共2页
Food and Drug
关键词
甘草黄酮
大孔树脂
分离
licorice flavonoid
macroporous resin
separation
