IL-24对氯化钴诱导缺氧乳腺癌细胞凋亡的影响

Effect of IL-24 on Cell Apoptosis of Hypoxia Breast Cancer Induced by Cobalt Chloride

为研究IL-24对缺氧条件下诱导肿瘤细胞凋亡的影响。体外培养乳腺癌细胞,诱导化学缺氧模型后,转染IL-24基因,通过流式细胞术分析细胞周期和凋亡。结果表明:与对照组比较,缺氧诱导后的细胞增殖速度减慢。流式细胞术双染法分析细胞凋亡,可发现在缺氧生长条件下,IL-24基因转染可诱导乳腺癌细胞凋亡,相同时间点,细胞凋亡率无显著差异。转染IL-24基因后,缺氧和非缺氧小鼠乳腺癌细胞的红色荧光强度均随时间延长而下降。IL-24可通过内质网途径诱导缺氧以及非缺氧小鼠乳腺癌细胞发生凋亡。

To analyze the effect of IL-24 on the induced tumor cell apoptosis under condition of hypoxia caused by cobalt chloride.Methods：The breast cancer cells were cultured in vitro and induced chemical anoxia models were set up.IL-24 gene transfection was carried out and cell apoptosis was analyzed by flow cytometry.The cell proliferation after hypoxia induction was slower compared with the control group.The breast cancer cell apoptosis happened after IL-24 genes transfection under condition of hypoxia by means of flow cytometry double staining.There was no significant difference of cell apoptosis rate at the same time point.The red fluorescence intensity of mouse breast cancer cells decreased with time under hypoxic and non-hypoxic conditions.IL-24 can induce the mouse breast cancer cell apoptosis under hypoxic and non-hypoxic conditions through the endoplasmic reticulum.