黄芪卫矛合剂调节糖尿病肾病大鼠HGF和TGF-β1平衡研究

The Balance of Diabetic Nephropathy Rat HGF and TGF- β Regulating by Huangqi Weimao Mixture

目的探讨黄芪卫矛合剂对糖尿病肾病大鼠的干预作用。方法用单侧肾切除并链脲佐菌素尾静脉注射建立大鼠糖尿病肾病模型,随机分为假手术组、糖尿病肾病组、糖尿病肾病+黄芪卫矛合剂组和糖尿病肾病+氯沙坦钾组。共持续12周,蛋白印迹法检测各组大鼠肾组织中肝细胞生长因子(HGF)和转化生长因子-β1(TGF-β1)蛋白表达。结果糖尿病肾病模型组HGF表达减少,TGF-β1蛋白表达增多,与假手术组比较均有非常显著性差异(P<0.01),两治疗组可增加HGF蛋白表达,降低TGF-β1蛋白表达,与模型组比较有显著性或非常显著性差异(P<0.01或P<0.05)。结论黄芪卫矛合剂能够通过增加糖尿病肾病大鼠肾组织中HGF蛋白表达,抑制TGF-β1蛋白表达,起到防治糖尿病肾病的作用。

Objective to explore the intervention effect of HQWM mixture on diabetic nephropathy rats. Methods use unilateral nephrectomy and streptozotocin injected into the tail vein to establish rat model of diabetic nephropathy, randomly divided into sham operation group, diabetic nephropathy group, diabetic nephropathy ＋ HQWM mixture group and diabetic nephropathy ＋ losartan potassium group. Total for 12 weeks, hepatocyte growth factor in renal tissue of rats in each group of Western blotting detection （HGF） and transforming growth factor beta 1 （TGF - β 1 ） protein expression. Results diabetic nephrepathy model group reduced expression of HGF, TGF - β 1 expression increased, compared with the sham operation groups were very significant difference （ P 〈 0.01 ）, two treatment group can increase the expression of HGF protein, decreased the TGF - β 1 expression, compared with model group, there were significant or very significant difference （ P 〈0.01 or P 〈 0. 05 ）. Conclusion HQWM mixture can increase the expression of HGF in renal tissue of diabetic nephropathy rats, inhibit the expression of TGF - β 1 expression, contributes to the prevention and treatment of diabetic nephropathy.