酶基抗生物粘泥复合膜的制备及性能

Preparation and properties of enzyme-based antifouling films

以多壁碳纳米管（MWNTs）和氧化石墨烯（GO）为载体，物理吸附制备固定化胰蛋白酶，并将固定化酶与聚甲基丙烯酸甲酯（PMMA）相结合制成具有催化活性的抗生物粘泥复合膜．对酶基抗生物粘泥复合膜的催化稳定性、抗蛋白质吸附性能、力学性能和抗菌吸附性能等方面进行了研究．复合膜在保存30d后仍具有90％左右的酶活性，且在重复使用6次后，酶活性损失率低于10％．在抗蛋白质吸附实验中，不合酶的膜表面蛋白质吸附量是含酶复合膜的4倍；将复合膜在革兰氏阴性菌和阳性菌悬浊液中分别培养一段时间，含酶复合膜表面附着的细胞个数比不含酶的膜表面少约1～2个数量级．

Trypsin was immobilized on the multiwall carbon nano-tubes （MWNTs） and graphene oxide （GO） by physical adsorption. The catalytic films were prepared by suspending the immobilized trypsin directly into a poly （methyl methacrylate） solution in toluene. These enzyme-based antifouling films can degrade the proteins which are the major components of slime. Some organic solvents such as acetone can improve the activity of the immobilized trypsin. The stabilities of MWNTs-Enzyme-PMMA film and GO-Enzyme-PMMA film stored at 25 ℃ and 50 ℃ were also studied. The results show that MWNTs-Enzyme-PMMA film can keep 90% of the initial activity after 30 days, while the residual activity of GO-Enzyme-PMMA film is 90% and 82% respectively. The two films also show good operational stabilities in six times of Continuous operations. Compared with the films without enzyme, MWNTs-Enzyme-PMMA film and GO- Enzyme-PMMA film can reduce the adsorption of BSA obviously. Additionally, the MWNTs-Enzyme-PMMA film and GO-Enzyme-PMMA film can also reduce the fouling of E.coli and S.aureus by two orders of magnitude.