模拟400m氦氧饱和潜水对大鼠抗氧化能力的影响

Effects of Simulated 400 m He-O_2 Saturation Diving on Antioxidative Capacity in Rats

[目的]观察模拟400 m氦氧饱和潜水对大鼠抗氧化能力的影响。[方法]将大鼠在加压舱4.1 MPa氦氧环境中饱和暴露24 h,以常压和4.1 MPa常氧高氦环境作为对照。出舱后测定大鼠肝、脑和肺中超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-px)活性和还原型谷胱甘肽(GSH)、丙二醛(MDA)及8-羟基脱氧鸟苷(8-OhdG)含量。[结果]4.1 MPa氦氧饱和暴露后大鼠脑SOD活性和肝、脑和肺GSH-Px活性降低(P<0.05),脑和肺GSH含量减少(P<0.05)。4.1 MPa常氧高氦暴露后大鼠各指标未见明显改变(P>0.05)。各组MDA和8-OhdG含量差异无统计学意义(P>0.05)。[结论]模拟400 m氦氧饱和潜水24 h使大鼠产生了氧化应激反应,抗氧化能力降低,其原因可能与该环境中的高分压氧有关。

[ Objective ] To observe the effects of simulated 400 m He-O2 saturation diving on the antioxidative capacity in rats. [ Methods ] Selected rats in an animal pressurized chamber were exposed to He-O2 for 24 h at 4.1 MPa, while normal air pressure and 4.1 MPa normoxic hyperbaric helium were used for two control groups. The activities of superoxide dismutase （SOD） and glutathione peroxidase （GSH-Px）, and contents of glutathione （GSH）, malondialdehyde （MDA）, and 8-Hydroxy-2＇-deoxyguanosine （8-OhdG） in liver, brain, and lung of exposed rats were determined. [ Results ] The activities of SOD in brain, the activities of GSH-Px in liver, brain, and lung, and the contents of GSH in brain and lung decreased in the 4.1 MPa He-O2 saturation group when compared with the two control groups （P 〈 0.05）. No differences were observed in all oxidative stress indices between the 4.1 MPa normoxic hyperbaric helium group and the normal pressure group （P〉 0.05）. There were also no differences in the contents of MDA and 8-OhdG among the three groups （P 〉 0.05）. [ Conclusion ] Simulated 400 m He-O2 saturation diving for 24 h can induce oxidative stress response and reduce antioxidative capacity in rats, which is probably caused by the high partial pressure of oxygen.