梅花鹿茸生物碱类成分及其对小鼠脾细胞增殖的影响

Alkaloids from Cervi Cornu Pantotrichum and Its Effect on Murine Splenocytes Proliferation

目的：研究梅花鹿茸生物碱类成分及其对小鼠脾细胞体外增殖的影响。方法：综合利用Sephadex LH-20和MCI（CHP20P）等柱色谱方法分离梅花鹿茸中的化学成分,运用多种波谱技术（UV、1H-NMR、13C-NMR和MS）鉴定化合物结构,应用MTT法考察梅花鹿茸总碱和8个生物碱成分对小鼠脾细胞体外增殖的影响。结果：从梅花鹿茸中分离得到11个化合物,分别鉴定为：尿嘧啶（1）、次黄嘌呤（2）、尿苷（3）、肌苷（4）、鸟苷（5）、2＇-脱氧鸟苷（6）、鸟嘌呤（7）、胸苷（8）、胸腺嘧啶（9）、胞苷（10）和腺苷（11）。小鼠脾细胞体外增殖活性研究发现梅花鹿茸总碱、尿嘧啶、腺苷具有明显的促进小鼠脾细胞增殖的活性。结论：其中,化合物4~11为首次从梅花鹿茸中分离得到,梅花鹿茸总碱是鹿茸免疫调节作用的物质基础之一,其中以尿嘧啶和腺苷活性最强。

Objective：To study the alkaloids of Cervi Cornu Pantotrichum and its effect on murine splenocytes proliferation. Methods ：The constituents isolation and purification from Cervi Cornu Pantotrichum was carried out by reported colunm chromatography including Sephadex LH-20 and MCI（CHP20P） and their structures were elucidated on the basis of spectral compounds. The method of MTT was used to examine the effects of eight alkaloids and total alkaloids content （ TAC ） of Cervi Cornu Pantotrichum on murine splenocytes proliferation. Results：Eleven compounds were isolated from Cervi Cornu Pantotrichum, and their structures were identified as follows：u- racil （ 1 ）, hypoxanthine （ 2 ）, uridine （ 3 ）, inosine （ 4 ）, guanosine （ 5 ）, 2 ＇-deoxygnanosine （ 6 ）, guanine （ 7 ）, thymidine （ 8 ）, thymine （ 9 ）, cytidine （10） and adenosine（ 11 ）. By the experiment of murine splenocytes proliferation activity in vitro, the results showed that the total alkaloids, uracil and adenosine had significantly promoted the proliferation of mouse spleen cells. Conclusion ： Compounds 4 - 11 are iso- lated from Cervi Cornu Pantotrichum for the first time. The total alkaloids is one of the material basis of immnnomodulatory effects of Cervi Cornu Pantotrichum,and uracil and adenosine are the most active.