基于色度对中药材品质评价研究(Ⅰ).黄连粉末色度与化学成分含量之间的相关性

Evaluation of Crude Drugs by Means of Colorimeter(Ⅰ).Study on Correlation Between Color and Alkaloids Content of Coptidis Rhizoma

目的:探讨基于传统中药理论色泽分辨黄连药材质量优劣及鉴别药材的方法。方法:采用测色计对黄连药材粉末进行色度测定,表述L*值、a*值、b*值,并用HPLC法测定黄连中小檗碱等6个生物碱的含量,对色度与生物碱含量之间的相关性进行分析。结果:黄连粉末粒度355μm以下,测定径:3 mm;观察视野:10°,F11光下粉末a*值与小檗碱含量(r=0.867,P<0.01)极显著相关;F2光及F7光下粉末a*值与总生物碱含量(r=0.793,P<0.05)显著相关。结论:黄连色度与生物碱含量之间具有相关性,采用测色计测定粉末色度可用于黄连品质的评价。

Objective：To examine the correlation between alkaloids content and L＊ ,a＊ and b＊ color indices of Rhizoma Coptidis for quality control. Methods：A colorimeter was used for the measurement of reflected light from sieved powder samples using the CIE 1976 L ＊ a ＊ b ＊ color system. The content of six alkaloids were determined by HPLC. The correlation between alkaloids content and color indices of Rhizoma Coptidis was analyzed. Results ： When the particle size of Rhizoma Coptidis was less than 355 μm and the colorime- ter parameters were set as measurement diameter of 3 mm, observation degree of 10, and light source of F2 and FT, the measured color was significantly correlated with total alkaloids content（r = O. 793, P 〈 0. 05 ）. As light source of F11, the measured color was signifi- cantly correlated with berberine content（ r = 0. 867 ,P 〈 0. 01 ）. Conclusion ：The correlation between the color of powdered Coptidis Rhizoma and its alkaloids contents was found in this study. Measurment of the color of Coptidis Rhizoma can be used to assess its quality.