多孔钛支架表面沉积牛血清白蛋白/钙磷生物活性涂层的构建

Fabrication of bovine serum albumin/calcium phosphate bioactive coating on porous titanium scaffolds

目的:通过仿生法在多孔钛支架表面构建含有牛血清白蛋白的钙磷涂层,评价牛血清白的整合对钙磷涂层形貌、化学成分的影响。方法:利用选择性激光熔覆方法制备多孔钛支架,将多孔钛支架经碱液处理后,浸入模拟体液中4d进行预钙化,随后将2组支架分别浸泡于含牛血清白蛋白浓度为10μg/mL的饱和钙磷溶液、不含牛血清白蛋白的饱和钙磷溶液中2d。检测支架表面涂层形貌及物相组成。结果:牛血清白蛋白加入后,钙磷涂层晶体尺寸变小,由微米级结构变为纳米级;涂层成分由羟基磷灰石和磷酸八钙混合相转变为碳酸化的羟基磷灰石。结论:成功在多孔钛支架表面构建了牛血清白蛋白/钙磷复合涂层;牛血清白蛋白整合到钙磷涂层内部。

Objectives： To fabricate apatite coating containing bovine serum albumin by co-deposition method on the surface of porous titanium scaffolds, and evaluate effects of incorporation of bovine serum albumin （BSA） on the morphology and chemical composition of the bioactive coating. Methods：The porous titanium scaffolds were prepared by using selective laser melting method. After alkali treatment, the porous titanium scaffolds were immersed into simulated body fluid for 4days for pre-calcification, subsequently two groups scaffolds were soaked in saturated calcium phosphate solution without or with containing BSA concentration of 10 μg/mL for 2 days. The topography of the specimen was observed by scanning electron microscopy （SEM）. Chemical structure and phase composition of coatings were detected by Fourier infrared spectroscopy （FTIR） analysis and X-ray diffraction （XRD） respectively. Results： the addition of bovine serum albumin reduced crystal size of calcium phosphate, changing from micron grade structure to nanoscale;and coating without BSA was composed of hydroxyapatite and octocalcium phosphate, while BSA/calcium phosphate coatings contained hydroxyapatite. Conclusion： We successfully fabricated hybrid coating of BSA/calcium phosphate coatings on porous titanium scaffolds, and crystal size of coating reduced with the addition of BSA .