摘要
目的研究模拟深埋条件下挤压伤复合低氧缺水缺食3 d大鼠解除挤压(即模拟"黄金抢救时间72 h")后即刻至28 d肝脏功能和结构的恢复规律与特点。方法应用雄性Wistar大鼠144只,随机分为对照组、单纯挤压组(单挤组)、低氧缺水缺食组(三缺组)、挤压伤复合低氧缺水缺食组(复合组),采用钳夹双后肢近端方法建立挤压伤模型[压力为(44.1±2.9)N]。各时间点每组6只,于解除挤压后即刻、1、3、7、14、28 d检测血清丙氨酸氨基转移酶(ALT)含量;切取肝脏,常规制片,光镜和电镜观察。结果①复合组1只大鼠于解除挤压后0.6 d猝死,其他3组未发生死亡。②复合组和单挤组ALT值于解除挤压后即刻明显升高,尤以复合组最显著(P〈0.01),3 d后均恢复正常。③复合组和单挤组肝质量均明显减轻,尤以复合组最显著(P〈0.05)。④复合组解除挤压后即刻和1 d肝索萎缩,肝细胞变性、凋亡,糖原减少,线粒体空化,并累及枯否细胞、储脂细胞、内皮细胞;3-7 d渐减轻,14-28 d未完全恢复。单挤组较之轻微。结论挤压伤大鼠在解除挤压后即刻肝脏功能和结构发生明显损伤,3~28 d渐恢复,具有病变累及全肝性、自行恢复性、功能与结构恢复不同步性和恢复程度差异性特点。
Objective To study the function and pathological changes of rat liver combined with hypoxia and deprivation of food and water for 3 days(" golden rescue time 72 h")under simulated condition of deep burial.Methods 144 male Wistar rats were randomly divided into four groups:control group,crush group,hypoxia combined with deprivation of water and food group(three-lack group),and crush injury combined with hypoxia and deprivation of water and food group(combined group).Crush injury model was established by clamping the near ends of double hind limbs [pressure for(44.1 ±2.9) N].Alanineaminotransferase(ALT) contents of serum were measured at 0,1,3,7,14 and 28 d after release from extrusion,and six animals for each group at every time.After histological section,the livers were observed by optical and electronic microscopy.Results ① In the combined group,one rat died at 0.6 d after release from extrusion.No death was seen in other three groups.② ALT values of combined group and crush group increased immediately release from extrusion,especially for the combined group(P〈0.01),and 3 days later both restored to normal.③The liver weight of combined group and crush group both reduced significantly,especially for the combined group(P〈0.05).④Liver atrophy,degeneration and apoptosis of liver cells,glycogen decrease and mitochondria cavitation was observed in combined group immediately and 1 d after release from extrusion,which involving kupffer cells,fat storing cells and endothelial cells.Above changes were alleviated gradually during 3- 7 d,but not fully recovered during 14- 28 d and mitigated in crush injury group.Conclusion Obvious functional and structural injury of rat liver was caused immediately after release from extrusion,which was alleviated gradually during 3- 28 d,and characterized as whole liver involved,restorative,different recover step and degree of function and structure.
出处
《军事医学》
CAS
CSCD
北大核心
2014年第6期417-421,共5页
Military Medical Sciences
基金
国家科技部709项目(2006FK130006)
关键词
模拟深埋条件
复合性挤压伤
大鼠
肝脏
解除挤压
simulant condition of deep burial
combined crush injury
rat
liver
release from extrusion