HPLC法同时测定五加芪菊颗粒中刺五加、菊花含量

Simultaneous Determination the Content of Acanthopanax, Chrysanthemum in Wujia Qiju Granule by HPLC

目的：建立高效液相色谱法同时测定五加芪菊颗粒中刺五加（以紫丁香苷计）和菊花（以3，5-O-二咖啡酰基奎宁酸计）含量的方法，为相关研究提供方法学参考。方法采用DiamonsilC18色谱柱（250 mm×4.6 mm，5μm），流动相：甲醇为流动相A，0.1%磷酸溶液为流动相B，进行梯度洗脱。检测波长为348 nm。结果紫丁香苷和3，5-O-二咖啡酰基奎宁酸的线性范围分别为0.10~40.0μg/mL（r=0.9984）、0.05~10.0μg/mL（r=0.9991），方法回收率分别为98.76%（n=6）、94.26%（n=6）。结论所建立的方法准确、灵敏度高、重现性好，在同一试验过程中，同时测定五加芪菊颗粒中刺五加、菊花含量。

ObjectiveTo establish a method for simultaneously determining the content of acanthopanax （as measured by syringin）, Chrysanthemum（as measure by （-）-3,5-Dicaffeoyl quinic acid） in Wujia Qiju granule.MethodsSimultaneously determined by HPLC on DiamonsilC18 （250 mm×4.6 mm,5 μm） by mobile phase of methanol （A） and 0.1% phosphonic acid solution （B） with gradient elution. Detection wavelength is 348 nm.ResultsThe linear concentration ranges of the calibration curves for syringin and （-）-3,5-dicaffeoyl quinic acid were 0.10~40.0 μg/mL（r=0.9984）,0.05~10.0 μg/mL（r=0.9991）, respectively. The average recoveries for syringin and （-）-3,5-dicaffeoyl quinic acid were 95.42% （n=6）,91.73%（n=6）, respectively.ConclusionThe method is accurate, highly sensitive and reproducible for simultaneously determination of content for acanthopanax and chrysanthemum in a single run by HPLC.