摘要
采用Sephadex LH-20色谱柱,以体积分数10%的甲醇为流动相分离人参中具有增强记忆活性的肽类成分,通过高效液相色谱系统将其纯化,并用蛋白质测序仪分析其氨基酸序列.结果表明,所得序列为Gln-Thr-Ser的新三肽.
The peptides, which show enhanced memory effect, from Panax a Sephadex LH-20 column, the mobile phase was volume fraction 10% of HPLC. Then the amino acid sequence was analysed by protein sequencer. ginseng were separated on methanol, and purified by The results of amino acid sequential analysis showed that a novel tripeptide whose amino acid sequence is Gln-Thr-Ser was obtained.
出处
《吉林大学学报(理学版)》
CAS
CSCD
北大核心
2014年第4期852-854,共3页
Journal of Jilin University:Science Edition
基金
国家自然科学基金(批准号:81173532)
吉林省科技发展计划项目(批准号:20130206046YY)
吉林省中医药管理局中医药科技项目(批准号:2012-114)
关键词
人参
氨基酸
序列分析
三肽
Panax ginseng
amino acid
sequential analysis
tripeptide