维吾尔族妇女宫颈病变组织p14^(ARF)表达与HPV基因分型的相关性研究

Study on the correlation between p14^(ARF) protein expression in cervical lesions and HPV genotyping in Uighur women in Xinjiang

目的:探讨新疆地区维吾尔族妇女不同程度宫颈病变组织p14ARF蛋白的表达及HPV感染情况。方法:运用免疫组织化学染色SP法检测120例维吾尔族妇女宫颈病变标本中p14ARF的表达情况,运用反向点杂交技术检测HPV分型情况。结果:随着宫颈病变程度的增加,p14ARF的表达出现升高趋势;慢性宫颈炎组与CINⅠ组、CINⅡ-Ⅲ组与SCC组比较,差异无统计学意义(P>0.05),其余各组两两比较,差异均有统计学意义(P<0.05)。不同级别宫颈病变组织HPV感染率差异明显(P<0.05),p14ARF蛋白在HPV感染组织中的表达明显高于未检测出HPV感染的组织,二者呈正相关(r=0.453,P<0.01)。结论:维吾尔族妇女宫颈病变及癌变组织p14ARF阳性表达随着病变级别的增加而增加,异常表达可能与感染HPV有关。

Objective： To explore the expressions of p14ARF protein in cervical lesions of different degrees and HPV infection in Uighur women in Xinjiang. Methods： Immunohistochemical SP method was used to detect the expression of p14ARF protein in cervical lesions samples in 120 Uighur women, reverse dot blot was used to detect HPV genotyping. Results： The expression of p14ARF protein showed an in- creasing trend with the aggravation of cervical lesions; there was no statistically signifieant difference between chronic cervicitis group and CIN I group, as well as between CIN Ⅱ-Ⅲ group and SCC group （P 〉 0. 05） , hut there was statistically significant difference between the other groups in pairs （ P 〈 0. 05 ） . There was statistically significant difference in HPV infection rate among tervital lesions of different de- grees （ P 〈 0. 05 ） , the expression level of p14ARF protein in cervical lesions with HPV inleetion was significantly higher than that in cervical lesions without HPV infection, there was a positive correlation between p14ARF protein expression and HPV intection（ r = 0. 453, P 〈 0. 01 ） . Conclusion： The positive expression rates of p14ARF protein in cervical lesions and cervical cancerization tissue of Uighur women increases with the aggravation of cervical lesions, abnormal expression of p14ARF protein may he correlated with HPV infection.