推拿对小儿哮喘慢性缓解期炎性细胞TLRs表达变化影响

Effect of massage on change of TLRs expressions in inflammatory cells of children with asthma during chronic remission phase

目的:观察推拿对小儿哮喘慢性缓解期中性粒细胞、肥大细胞、巨噬细胞TLR1、TLR2、TLR4表达的影响。方法:将慢性缓解期哮喘患儿各100例,严格遵循随机分组的原则分为治疗组50例和对照组50例。对照组采取常规的预防措施,治疗组在常规治疗的基础上,应用小儿推拿治疗28天,在治疗前、治疗后取小儿外周血,检测中性粒细胞、肥大细胞、巨噬细胞TLR1、TLR2、TLR4的表达。结果:治疗后治疗组中性粒细胞、肥大细胞、巨噬细胞TLR1、TLR2、TLR4表达荧光强度与对照组比较,有统计学差异(P<0.05)。结论:小儿推拿手法可以通过上调中性粒细胞、肥大细胞、巨噬细胞TLR1、TLR2、TLR4表达来改善小儿哮喘的临床症状。

Objective： To observe the effect of massage on TLR1, TLR2 and TLR4 expressions in neutrophile granulocytes, masto- cytes and macrophages of children with asthma during chronic remission phase. Methods： One hundred children with asthma during chronic remission phase were randomly divided into treatment group and control group, 50 children in each group. The children in control group were treated with conventional preventive measures ; the children in treatment group were treated with massage for 28 days based on conventional therapy; peripheral blood samples of the children were obtained before and after treatment to detect the expressions of TLR1, TLR2 and TLR4 in neutrophile granulocytes, mastocytes and macrophages. Results： There were statistically significant differences in the expression levels of TLR1, TLR2 and TLR4 in neutrophile granulocytes, mastocytes and macrophages after treatment between treatment group and control group （P 〈 0. 05 ） . Conclusion： Massage can improve clinical symptoms of asthma in children by upregulating the expressions of TLR1, TLR2 and TLR4 in neutrophile granulocytes, mastocytes and macrophages.