表柔比星-白藜芦醇复方脂质体的制备及体外抗肿瘤作用研究

Preparation of Epirubicin-Resveratrol Compound Liposomes and Its Anticancer Effects in Vitro

目的对表柔比星-白藜芦醇复方脂质体的制备工艺进行研究,并对其抗肿瘤作用进行评价。方法采用薄膜分散法-硫酸铵梯度法制备复方脂质体,并对处方进行优化;用RP-HPLC测定复方脂质体的包封率;磺酰罗丹明B蛋白染色法(sulforhodamine B,SRB)评价体外抗肿瘤作用。结果采用最优处方,以薄膜分散法-硫酸铵梯度法制备的复方脂质体中表柔比星与白藜芦醇包封率分别为87%、61%;制备得到的复方脂质体对C6胶质瘤细胞具有较强的抑制作用。结论本方法制备复方脂质体操作简便、重复性好,包封率较高,体外抗肿瘤作用强。

OBJECTIVE To study the preparation process of epirubicin-resveratrol compound liposomes （EPI-RES-L） and inves- tigate its anticancer effects in vitro. METHODS The compound liposomes were prepared by film dispersion-pH gradient method. The formulation was optimized, and the encapsulation efficiency was determined by RP-HPLC. The anticancer effect of EPI-RES-L in vitro was studied by sulforhodamine B （SRB） protein staining method. RESULTS The encapsulation efficiencies were 87% and 61% for epirubicin （EPI） and resveratrol （RES）, respectively. The cytotoxieity of EPI-RES-L was significantly higher than that of free drugs against C6 glioma cells. CONCLUSION The film dispersion-pH gradient method is simple, reproducible, and has high drug encapsulation efficiency. EPI-RES-L has potent anticancer effect.