HPLC法测定不同产地火麻仁药材中胡芦巴碱的含量

Determination of trigonelline in fruit of Cannabis sativa from different origin by HPLC

目的：建立高效液相色谱测定火麻仁药材中胡芦巴碱含量的方法，为2015版药典火麻仁指标性成分的含量测定项提供依据。方法：采用Hypersil氨基柱，流动相乙腈－水（80∶20，V∶V），检测波长265nm。结果：胡芦巴碱进样量在0.104-1.04μg范围内线性关系良好，r=0.9999（n=6），加样回收率100.7%，RSD=1.56%（n=9）；10批火麻仁药材中胡芦巴碱的含量为0.6504～0.9931mg·g^-1。结论：胡芦巴碱为火麻仁药材及饮片中的特征成分，其提取及含测方法操作简便，结果可靠，可作为火麻仁药材质量控制的指标性成分。

Objective： An HPLC method for determination of trigonelline in fruit of Cannabis sativa was developed, to provide a quality control method for 2015 edition of Chinese Pharmacopoeia. Methods： The chromatogra-phy was carried out on a Hypersil amino column（250mm×4.6mm, 5μm）with acetonitrile-water（80：20, V：V）as the mobile phase, the detection wavelength was set at 265nm. Results： Trigonelline could be successfully deter-mined and the linear rang was 0.104-1.04μg （r=0.9999）. The average recoveries （n = 9）of the trigonelline was 100.7%,RSD=1.56%. The content of trigonelline in 10 batches was 0.6504-0.9931mg ·g^ -1. Conclusion：Trigonelline was indeed the specific ingredient in fruit and seed of Cannabis sativa. The method is accurate, reli-able and sensitive for quality control of fruit of cannabis sativa.