丹参酮ⅡA磺酸钠对肢体缺血再灌注后大鼠肺组织超微结构的影响

Effect of sodium tanshinone ⅡA silate on ultra-microstructure of lung tissue in rats with limb ischemia-reperfusion injury

目的探讨丹参酮ⅡA磺酸钠对大鼠肢体缺血再灌注后肺组织超微结构的影响。方法将60只雄性SD大鼠随机分成假手术组(A组)、单纯缺血再灌注组(B组)和丹参酮ⅡA磺酸钠组(C组)。采用常规法建立大鼠肢体缺血再灌注损伤模型。利用光镜和电镜观察肺组织形态学改变和超微结构改变,利用比色法测定肺组织中MDA、SOD、XOD水平,并进行比较。结果与B组相比,C组肺血管扩张、炎性细胞聚集、组织损伤明显减轻;肺组织MDA、XOD活力明显降低,SOD活力升高。结论丹参酮对肢体缺血再灌注后肺组织结构具有明显的保护作用。

Objective It is to explore the effect of sodium tanshinone ⅡA silate on ultra-microstructure of lung tissue in rats with limb ischemia-reperfusion injury .Methods 60 male SD rats were randomly divided into sham operation group （ group A）, simple ischemia-reperfusion group （group B） and sodium tanshinone ⅡA silate group （group C）.Limb ischemia-reper-fusion injury models of the rats were established by normal method .The histological and ultra-structure changes were observed under light microscope and electron microscope respectively .The levels of MDA , SOD and XOD in lung tissue of every group were determined by colorimetric method .Results Compared with that in group B , pulmonary vascular dilatation , inflammatory cell aggregation and tissue injury were obviously improved in group C , the activities of MDA , XOD were lower and that of SOD was higher in group C .Conclusion Sodium tanshinone IIA silate has an obvious protecting effect on lung tissue structure after limb ischemic-reperfusion .