耐盐高降解蛋白菌株的分离鉴定及其降解条件的研究

Identification and study on degradating condition of halophilous protein degradation strain Serratia sp.A3

通过耐盐实验和发酵实验,从餐饮废水中分离得到一株耐盐且高效降解蛋白的细菌(A-3),为了确定该菌株的最佳降解条件,选择培养基初始pH、溶氧量、接种量和碳源4个影响因素进行降解实验。通过形态学、生理生化特征以及16S rDNA鉴定,将A-3初步鉴定为沙雷氏菌(Serratia sp.A3)。蛋白质降解实验结果表明:耐盐降解蛋白菌Serratia sp.A3最佳降解条件为:初始pH7.0,溶氧量180r/min,接种量5%,蔗糖作为碳源可以促进菌株对蛋白质的降解。

A halophilous protein degradation bacteria A-3 was isolated from the restaurant wastewater by salt tolerance test and fermentation test.In order to determine the optimum conditions for the degradation of the strain, Four influencing factors such as initial pH, dissolved oxygen,inoculum size and carbon source were selected do degradation inoculation experiments. Based on morphologic observation, biochemical characteristic and identification of 16S rDNA,A-3 belonged to the genus of Serratia sp.A3.The results showed that.The strain Serratia sp.A3 optimum degradation environmental conditions was pHT.O, dissolved oxygen 180r/min,5% inoculum size, the Sucrose could promote the protein degradation of Serratia sp.A3.