基于MonoTrap捕集法检测中华绒螯蟹性腺和肝胰腺中的香气成分

Comparison of volatile compounds in Chinese mitten crab(Eriocheir sinensis) hepatopancreas and crab gonad by monotrap adsorption

采用新型材料——MonoTrap作为固相萃取整体捕集剂,提取中华绒螯蟹的挥发性成分。利用气相色谱-质谱联用仪(GC-MS)对雌性中华绒螯蟹生/熟两个部位(肝胰腺和性腺)中的挥发性成分进行了分离鉴定,共得到8大类74种挥发性物质。对这些物质进行显著性差异分析和主体呈香物质的分析,并对其产生途径进行了探讨。结果表明:肝胰腺和性腺中的挥发性物质具有显著性差异,且生样蒸熟的过程中产生了大量的醛类、酮类、醇类、呋喃类、含氮类化合物,推断这些物质是在加热过程中由风味前体物质反应或分解生成。在主体呈香物质中,癸醛、三甲胺、壬醛为生/熟肝胰腺和性腺所共有的,而3-甲基丁醛、辛醛则是熟制样品特有的挥发性物质,此外,熟制肝胰腺中含有独特的2-甲基丁醛。

To investigate the different volatile compounds in two edible parts （hepatopancreas and gonad）of raw and cooked female Chinese mitten crab（ Eriocheir sinensis）cultured in Yangcheng Lake,they were extracted by a new type material （MonoTrap） and analyzed by gas chromatography- mass spectrometry （ GC- MS）. Totally 74 volatile compounds were identified.The peak areas were analyzed by significant analysis, and then the odor-active compounds were selected based on ROVA values. There was a significant difference between the volatile compounds of hepatopancreas and gonad. A large number of aldehydes, ketones, alcohols, furans and N- containings were produced in steaming process. Trimethylamine, nonanal, decanal were the main odor-active compounds in raw/cooked hepatopancreas and gonad, while 3-methylbutanal and octanal were only found in cooked samples. Also, 2- methylbutanal was the main odor- active compounds only found in cooked hepatopancreas.