食源性大肠杆菌PCR检测技术的研究进展

Research progress in PCR detection for foodborne Escherichia coli

大肠杆菌主要附着在人或动物的肠道内,属于正常菌群。食品中检出大肠杆菌意味着食品直接或间接的受到了粪便的污染。为了保障食品的食用安全性,需要对食品中的大肠杆菌进行检测。传统的检测方法存在耗时长、灵敏度低等问题,PCR技术可以很好的解决这些问题。PCR又称作聚合酶链式反应(Polymerase Chain Reaction),该方法是在体外快速扩增特定基因或DNA序列,从而对病原微生物进行快速检测的方法。PCR技术因具有快速、特异性高、成本低等优点而广泛的应用于快速检测中。本文对应用在食源性大肠杆菌上的几种主要的PCR检测技术的研究现状进行综述。

Escherichia coil is naturally found in the intestinal tracts of all warm-blooded animals,including humans. The presence of Ecofi in food or water means that the food or drinking water may directly or indirectly polluted by manure.In order to ensure food safety, it is necessary to detect E. coil in food.The traditional detection methods have some problems, such as time-consuming, low sensitivity, and so on. PCR technology can solve these problems well.The PCR technique is a rapid amplification of specific genes or DNA sequences,which can detect pathogenic microorganisms rapidly.Because PCR technology has the advantages of high speed, high specificity and low cost,it is widely applied in rapid detection.In this paper,the application of several main PCR technologies on foodborne Ecofi was summarized.