牡蛎提取物持钙性能研究

Study on absorption promottion of calcium activity extracts from oyster

以湛江牡蛎为原料,重点对其水提物持钙性能进行研究。结果表明,牡蛎提取物在添加浓度为600mg/L时,沉淀平衡时间可高达20min。当添加浓度在100mg/L以上时,酪蛋白磷酸肽(CPP)延长磷酸钙的平衡时间远远高于牡蛎水提物,而在100mg/L以下时,两者延长磷酸钙的平衡时间基本一致。随着添加物浓度的增加,其络合钙的能力明显增强,200mg/L的牡蛎水提物可使磷酸钙中的Ca溶出量达到29.6mg/L,在添加浓度小于200mg/L时,牡蛎水提物的钙络合能力要低于CPP阳性对照,但当添加浓度大于200mg/L时,牡蛎水提物的钙络合能力已经超出CPP。牡蛎胃蛋白酶酶解物的持钙活性最强,通过二次酶解试验和全波长扫描图推测,其功能成分可能是蛋白源的多肽。

Taking Zhanjiang oysters as material, the promote calcium absorption activities of aqueous extracts from oysters were mainly researched. The results showed that with the concentration of oyster extract additon to 600 mg/L, the equilibrium time can be up to pre- cipitation 20 min; When the addition was 100 mg/L or more, CPP extension of time was much higher than the balance of calcium phosphate aqueous extract of oysters; But when addition concentration was 100 mg/L or less, both of the balance time was the same. With increasing concentration of the additives, the ability to complex calcium was significantly enhanced, and the dissolving content of the calcium phosphate Ca reached 29.6 mg/L in 200 mg/L of water extract of oyster; When the addition, concentration was less than 200 rag/L, the calcium complexation ability of aqueous extract of oysters CPP was lower than the positive control, but higher than with the concentration over 200 mg/L. Held calcium activity oyster hydrolysates by pepsin has the strongest calcium holding activity Through secondary digestion experiments and full wavelength scans the function may be polypeptide protein.