蒲桃茎化学成分及其体外细胞毒活性研究

Chemical constituents from stems of Syzygium jambos var. jambos and their in vitro cytotoxicity

目的研究蒲桃(原变种)Syzygium jambos var.jambos茎的化学成分。方法利用硅胶、葡聚糖凝胶、氧化铝和制备液相等各种色谱技术进行分离和纯化,根据波谱数据分析鉴定化合物结构;利用MTT法评价分离化合物对人肝癌细胞Huh-7细胞毒作用。结果从蒲桃茎醋酸乙酯和甲醇提取物中分离得到12个化合物,分别鉴定为麦珠子酸(1)、urolithin A(2)、邻苯二甲酸二正丁酯(3)、桦木酸(4)、熊果酸(5)、1-(4-methoxyphenyl)-1,2-propanediol(6)、2,6-dimethoxy-1,4-benzoquinone(7)、邻苯二甲酸二异丁酯(8)、β-谷甾醇(9)、3-乙酰-熊果酸(10)、积雪草酸(11)和阿江榄仁酸(12)。细胞活性测试结果表明在50μmol/L浓度时化合物1、7、10、11+12混合物对Huh-7细胞均未表现出显著的细胞毒作用。结论除化合物4,其他11个化合物均为首次从植物分离获得,所测试化合物均无明显细胞毒活性。

Objective To study the chemical constituents from the stems of Syzygium jambos var. jambos. Methods The phyotchemicals were isolated by using various chromatographic techniques such as silica gel, Sephadex LH-20, alumina column chromatography, and preparative HPLC process. The structures were elucidated on the basis of spectral data analysis. Their cytotoxicity against human liver cancer cells Huh-7 was conducted using MTT assay. Results Twelve compounds were isolated from the EtOAc and MeOH extracts in the stems of S. jambos and identified as alphitolic acid （1）, urolithin A （2）, dibutyl phthalate （3）, betulinic acid （4）, ursolic acid （5）, 1-（4-methoxyphenyl）-l, 2-propanediol （6）, 2, 6-dimethoxy-1, 4-benzoquinone （7）, diisobutyl phthalate （8）, β-sitosterol （9）, 3-acetyl-ursolic acid （10）, asiatic acid （11）, and arjunolic acid （12）, respectively. All tested compounds 1, 7, 10, and 11 ＋ 12 mixture did not show the cytotoxic activities towards human tumor cells Huh-7 in the test range t!p to 50 μmol/L. Conclusion Except compound 4, the other 11 compounds are obtained from this plant for the first time. All tested compounds exhibit no significant cytotoxic activity against Huh-7 cells.