摘要
目的建立同时测定人参及其制剂中16种人参皂苷的HPLC方法。方法采用C18(150mm×4.6mm,5μm)色谱柱;流动相为乙腈和水,梯度洗脱,体积流量1.0mL/min,检测波长203nm,柱温35℃。结果16种人参皂苷Rgl、Re、Rf、Rbl、Rg2、Rc、Rb2、Rb3、F1、Rd、F2、Rg3、Rh2及原人参三醇、compoundK、原人参二醇均得到良好分离,线性关系良好(r≥0.9990)。加样回收率均在95%-102%,RSD〈2%。结论该方法快捷简便、稳定可靠,可应用于人参及其制剂的质量控制。
Objective In order to evaluate the quality of Panax ginseng and its preparation, a simple and accurate HPLC method for determining the contents of 16 ginsenosides from P ginseng was established. Methods The chromatographic separation was achieved on a C18 column (150 mm ×4.6 mm, 5μm) using a mobile phase made up of acetonitrie and water at a flow rate of 1.0 mL/min. The detection wavelength and column temperature were set as 203 nm and 35 ℃, respectively. Results Sixteen ginsenosides (Rgl, Re, Rf, Rbl, Rg2, Rc, Rb2, Rb3, F1, Rd, F2, Rg3, protopanaxatriol, compound K, Rh2, and protopanaxadiol) were separated at baseline with good linearity (r 〉 0.999 0). The recovery rates were 95%-102% (RSD 〈 2%). Conclusion The method is simple, fast, accurate, and could be applied to the quality control of P. ginseng and its preparation.
出处
《中草药》
CAS
CSCD
北大核心
2014年第14期2009-2013,共5页
Chinese Traditional and Herbal Drugs
基金
国家科技支撑计划项目(2011BAI03B010602)
国家公益性行业科研专项(201303111)
国家科技重大专项子课题(2012ZX09304006)
吉林省基础研究项目(20130102075JC)
吉林省科技厅重点项目(20110228)
吉林省科技条件与平台建设计划(20112101)
吉林省现代农业产业技术体系建设项目(201218)