小鼠高海拔运动下负载量与内分泌紊乱关系建模

Modeling Relationship Between Load and Eendocrine Disorder Under Mice High Altitude Movement

目的:分析高海拔运动下负载量同内分泌紊乱关系。方法:通过低压低氧舱模拟海拔5000 m高原低氧环境复制动物模型,将小鼠划分成常氧对照组以及低氧组:低负载量、中负载量以及高负载量组。测定小鼠血清游离三碘甲腺原氨酸FT3和促甲状腺激素FT3水平,分析高海拔运动下负载量逐渐增加过程中小鼠内分泌指标变、小鼠血浆中雌二醇水平,小鼠身体做功能力以及最大耗氧量情况。结果:随着低氧运动负载量的不断增加,低氧运动组小鼠体重都显著低于对照组,与常氧对照组对比,低氧运动的血清果酮下降(P>0.05),低氧中负载运动(P<0.01)和低氧高负载运动(P<0.05)血清皮质醇呈现下降趋势,T/C在低氧低、中、高负载量(P<0.05、P<0.01、P<0.05)较常氧对照组显著升高,低氧运动组小鼠血清下丘脑-垂体-游离三碘甲腺原氨酸FT3和促甲状腺激素TSH不断降低,并且运动负载量越大,小鼠FT3和TSH越低,运动后小鼠血浆雌二醇水平均较运动前显著降低(P<0.01或P<0.05)并且随着运动负载量的增加,低氧运动组小鼠的血浆雌二醇水平呈现下降趋势。小鼠机体出现适应变化、机体做功能力以及最大耗氧量都降低。结论:说明高海拔低氧运动下,运动员的运动负载量越高,其机体内分泌各指标值越低,内分泌系统活力大大降低,小鼠内分泌的紊乱程度增大,使得小鼠机体功能大大减弱。

Objective：Using ELISA method and determination of mice serum free the iodine armour gland original FT3 and thyroid-stimulating hormone FT3 level, amino acid analysis of high altitude movement under load in the process of gradually increasing in the endocrine change in mice, mice plasma estradiol levels, physical power capability and maximum oxygen consumption in mice. Results： As the hypoxic exercise load increasing, hypoxia group exercise mice, the weight was significantly lower than the control group, compared with often oxygen control group, the serum fruit of hypoxic exercise decreased （ P〉0.05）, low oxygen in load exercise （P〈0.01） and low oxygen and high load exercise （P〈0.05） in serum cortisol showed a trend of decline, T/C in the low, low oxygen and high load （P〈0.05,P〈0.01,P〈0.05） than oxygen was significantly elevated, hypoxic exercise group mice serum hypothalamic- pituitary- free three iodine armour gland original glycine, decrease of FT3 and thyroid-stimulating hormone TSH and exercise load, the greater the FT3 and TSH is lower, in mice after exercise mice serum estradiol levels＆amp;nbsp;than before exercise significantly reduced （P〈0.01 or P〈0.05） and with the increase of motor load, low oxygen movement presents downtrend plasma estradiol levels of mice. The body adapt to change, the body＆#39;s ability to work in mice and maximum oxygen consumption are reduced. Conclusion： Under high altitude hypoxia exercise, the higher the load of the movement of athletes, the lower the body＆#39;s endocrine each parameter values, the endocrine system vitality greatly reduced, the endocrine disorder degree increase in mice, greatly weakened the body function in mice.