斑马鱼外周血液及造血器官血细胞发生的观察

Development of Haemocytes in Peripheral Blood and Hematopoietic Organs of Zebra Fish,Danio rerio

通过对斑马鱼外周血涂片以及肝脏、肾脏和脾脏的组织印迹片进行Wright’s-Giemsa混合染色,光镜观察其中各种血细胞的大小、形态特征,并对血细胞进行分类统计,研究了斑马鱼外周及造血组织血细胞的发生过程。研究发现,斑马鱼的血细胞除红细胞外,还包括单核细胞、淋巴细胞、嗜中性粒细胞、少量的嗜酸性粒细胞、嗜碱性粒细胞和血栓细胞。斑马鱼血细胞的发育大致经过3个阶段,即原始阶段、幼稚阶段和成熟阶段。造血母细胞从造血器官释放入外周血中有一个成熟过程。原始红细胞只在肾脏中出现,而肾脏中幼稚型红细胞的数目也最多。原始粒细胞、原单核细胞和原淋巴细胞在3种器官中均有出现,肾脏最多。可见肾脏是斑马鱼主要造血器官,脾脏和肝脏是其辅助造血器官。

The size, morphological characteristics and percentages of blood cells and patterns of developments of blood cells were ultimately observed in whole blood, kidney, liver and spleen by Wright＇s-Giemsa smears in order to investigate the development of haemocytes in peripheral blood and hematopoietic organs in zebra fish Danio rerio. Many kinds of leukocytes were found in the peripheral blood and hematopoietic organs, including monocytes, lymphocytes and neutrophils, and a small amount of eosinophilic granulocytes, basophilic cells and thrombocytes as well as erythrocytes. The development of blood cells in zebra fish was divided into three stages：primitive stage, immature stage and mature stage. The process of maturation was involved in flowing of parent cells from hematpopietic organs into the peripheral blood vessels. The primitive erythrocytes were only found in the kidney, and the immature erythrocytes were also very rich in the kidney. The primitive cells of monocyte, lymphocyte and granulocyte were appeared in all three organs, and most were found in kidney. The findings showed that kidney was of the principal hematopoietic organ, spleen and liver being auxiliary organs in zebra fish.