松茸子实体石油醚部位化学成分研究

Chemical constituents of petroleum ether extract from fruit bodies of Tricholoma matsutake

目的:研究松茸石油醚部位的化学成分及主要脂肪酸的种类与含量,为更好地开发利用松茸资源提供依据。方法:利用色谱技术对松茸干燥子实体的石油醚提取部位进行化学成分的分离纯化,通过波谱解析确定其结构。通过甲酯化,运用GC-MS技术测定其脂肪酸组成与含量。结果:分离得到5个甾体类化合物,经理化及波谱数据分析鉴定为麦角甾醇过氧化物(Ⅰ)、麦角甾醇(Ⅱ)、麦角甾-7,22-二烯-5,6-环氧-3β-醇(Ⅲ)、麦角甾-7,22-二烯-3β-醇(Ⅳ)、麦角甾-7,22-二烯-3β,5α,6β-三醇(Ⅴ)。从松茸的脂肪中检出6种主要脂肪酸,以油酸和亚油酸为主,其相对含量分别为57.6%和26.7%。结论:除化合物Ⅱ外均为首次从松茸子实体中分离鉴定。

OBJECTIVE To study the chemical constituents of petroleum ether extract, to determine the main fatty acids inthe fruiting bodies of Tricholoma matsutake and provide the basis for better development and utilization of Tricholoma matsutake sources. METHODS The compounds were isolated with silica gel column chromatography and their structures were elucidated on the basis of chemical evidences and spectral analysis. After methyl esterized, its fatty acids composition were analyzed by gas chromatography-mass spectroscopy （GC/MS）. RESULTS Five compounds were isolated and identified as ergos- terol peroxide（ Ⅰ ）, ergosterol（Ⅱ）, ergosta-7,22-dien-5,6-epoxy -3-o1（ Ⅲ ）, ergosta-7,22-diene-313-ol （ Ⅳ ） and ergosta-7,22- diene-3β, 5α, 6β-triol（ V ）. Six kinds of fatty acids were identified and the oleic acid （57. 6%） and linoleic acid（26. 7%）were the main components among them. CONCLUSION Compounds were firstly isolated from the fruiting bodies of Trieholoma matsutake except for compound II.