钩状石斛组织培养技术研究

Study on Aseptic Tissue Culture Technique of Dendrobium aduncum

[目的]解决钩状石斛的快速繁殖和提高组培苗成活率。[方法]在基本培养基MS无菌播种下,获得大量无菌幼苗,增殖过程分别采用不同配比的植物生长激素6-BA和NAA组合,筛选出钩状石斛无菌幼苗增殖最佳浓度配比;壮苗生根培养过程中采用不同浓度的植物生长激素IBA和IAA,观察不同因素对生根的影响。移栽技术研究,采用3种不同基质花生壳、刨花和树皮椐末混合,观察不同基质对钩状石斛组培生根苗移栽成活率的影响。[结果]钩状石斛种子萌发最佳配方:MS+6-BA 0.5 mg/L+香蕉10%+白糖20 g/L+琼脂5 g/L+AC 2 g/L。钩状石斛幼苗增殖最佳培养配方:MS+6-BA2 mg/L+NAA 1 mg/L+香蕉10%+白糖20 g/L+琼脂5 g/L。钩状石斛壮苗生根最佳培养配方:1/2MS+IBA 2 mg/L+香蕉10%+白糖20 g/L+琼脂5 g/L+AC 2 g/L。3种基质均对钩状石斛组培苗根生长情况和苗的成活率都有明显的促进作用,其中花生壳为最适宜基质。[结论]该研究为以后工业化快速生产钩状石斛,及钩状石斛的合理开发和可持续利用提供参考。

[Objective] To study on rapid propagation technique and the soma clone survival rate of Dendrobium aduncum.[Method] A large number of aseptic seedlings produced from culture of MS,different ratio of 6-BA and NAA were tested to obtain the best media for multiplication ;in process of rooting,adding different concentration of IBA and IAA into the basic culture medium to study the effects on radiation.To research the survival rate of seedlings of Dendrobium aduncum through adding peanut shall and shavings and bark to different medium.[Result] The suitable medium for seed germination was MS ＋ 6-BA0.5 mg/L ＋ banana10％ ＋ white sugar 20 g/L ＋ agar 5 g/L ＋ AC 2 g/L.The best medium for multiplication of seedlings was MS ＋6-BA 2 mg/L ＋ NAA 1 mg/L ＋ banana 10％ ＋ white sugar 20 g/L ＋ agar 5 g/L.The optimal medium for rooting was 1/2 MS ＋ IBA 2 mg/L ＋ banana 10％ ＋ white sugar 20 g/L ＋ agar 5 g/L ＋ AC 2 g/L.The most medium for transplanting：All three kinds of medium could remarkably accelerate root growing,the length and survival rates of plantlets.The plantlets were transplanted into peanut shall medium resulted highest survival rates.[Conclusion] This study provided reference for industrial production and the development and sustainable use of Dendrobium aduncum.