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cDNA-AFLP技术及其在基因差异表达中的应用 被引量：4

cDNA-AFLP Technology and Its Application in Gene Differential Expression
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摘要 cDNA-AFLP技术是一种在转录水平上研究基因差异表达的分子生物学实验技术,由于其具有起始材料量少、假阳性低、重现性、灵敏度高且不需预先知晓序列信息等优点,被广泛应用于各类生物差异表达基因的研究。就cDNA-AFLP技术的基本原理、发展历史和技术特点以及在微生物研究尤其是乳酸菌发酵研究中的潜在应用价值进行了综述。 cDNA-AFLP is a kind of molecular biological experimental technology which detects differential expression of genes at the transcriptional level.Due to its little material,low false positive,high repeatability,high sensitivity and less sequence information is widely used in various types of studies of biological gene differential expression.The basic principle,development history,technical characteristics and the potential application of cDNA-AFLP technology in lactic acid bacteria fermentation was discussed.
作者 王超 曲晓军 崔艳华
机构地区 哈尔滨工业大学食品科学与工程学院 黑龙江省科学院微生物研究所
出处 《安徽农业科学》 CAS 2014年第21期6937-6940,共4页 Journal of Anhui Agricultural Sciences
基金 国家自然科学基金资助项目(30901048)
关键词 CDNA-AFLP 基因差异表达 技术特点 应用 cDNA-AFLP Differential gene expression Technical characteristics Application
分类号 S188 [农业科学—农业基础科学]
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安徽农业科学
2014年 第21期

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