超高效液相色谱-串联质谱法测定糕点中富马酸二甲酯

Determination of Dimethyl Fumarate in Cake by Ultra High Performance Liquid Chromatography-Tandem Mass

[目的]简便快捷地测定糕点中的防腐剂富马酸二甲酯。[方法]建立了一种使用岛津超高效液相色谱仪LC-30A和三重四级杆质谱仪API 4000联用测定食品中富马酸二甲酯含量的分析方法。[结果]试验表明,富马酸二甲酯标液的线性关系为y=0.972 7x+0.001 2,线性相关系数为0.999 4。采取加标的方式进行方法验证,对同一富马酸二甲酯浓度的加标样品进行6次精密度试验,富马酸二甲酯的回收率范围为98.4%～101.5%,相对标准偏差RSD为1.2%,方法检出限为0.01 mg/L。[结论]该方法准确、快速、操作简便,可用于糕点中富马酸二甲酯的定量分析。

[Objective] To rapidly and easily determine dimethyl fumarate in cake.[Method] UHPLC LC-30A and API 4000 triple quadrupole mass spectrometer was established for determination of dimethyl fumarate content.[Result] The results showed that a linear relationship dimethyl fumarate was y =0.972 7x ＋ 0.001 2,the linear correlation coefficient of 0.999 4.Six times precision experiments were conducted on the same concentration of dimethyl fumarate.The range of recovery rate is 98.4％-101.5％,RSD is 1.2％,detection limit is 0.01 mg/L.[Conclusion] The method is accurate,rapid and simple,which can be used in quantitative analysis of dimethyl fumarate.