摘要
目的通过动物试验观察阿维菌素的亚急性毒性,从氧化性损伤角度探讨其毒作用机制,为接触阿维菌素的职业人群的疾病防护提供依据。方法将健康Wistar大鼠随机分为3组,每组10只,雌雄各半,分别为高剂量组(1/4 LD50)、低剂量组(1/8 LD50)和阴性对照组,采用经口灌胃方式连续染毒30 d。每周称量动物体重,实验结束后腹腔注射戊巴比妥钠麻醉动物,腹主动脉采血,检测血清MDA含量及GSH-Px、SOD活性;取动物肝、脾、肾、睾丸(或卵巢)称重,计算脏体比,组织用10%甲醛溶液固定,用于病理组织学检查。结果染毒后,阿维菌素高、低剂量染毒组与对照组相比,大鼠体重的增长幅度均呈现出降低趋势,实验结束时,三组雌性大鼠体重差值差异有统计学意义(F=102.85,P<0.05);高剂量染毒组雌性大鼠的体重低于阴性对照组(t=3.235,P<0.05);雌性大鼠的脑体比、肝体比、肾体比在三组间比较,差异有统计学意义(F=22.34、93.15、60.77,P均<0.05);雌性高剂量组与对照组的脑、肝、肾体比差异,雌性低剂量组与对照组肝体比差异,雌性高剂量组与低剂量组脑体比的差异均有统计学意义(t=13.69、20.14、16.20、8.91、8.36,P均<0.05)。大鼠血清MDA含量及GSH-Px、SOD活性在三组间比较,差异均有统计学意义(F=57.48、120.62、30.45,P均<0.05)。与对照组相比,阿维菌素组大鼠MDA含量显著增加,GSH-Px、SOD活性显著降低(P<0.05)。病理学结果显示:阿维菌素高、低剂量组大鼠肝组织个别汇管区均见少量单个核细胞浸润,并且在其周围发现少许轻微萎缩的肝细胞,其他脏器未见异常。结论阿维菌素可降低大鼠体重,对大鼠肝脏造成损伤,且氧化性损伤是阿维菌素毒作用机制之一。
Objective Subacute toxicity of abamectin was studied and the oxidative damage was reported. Methods A total of 30 healthy Wistar rats(15 male and 15 female) were randomly divided into 3 groups,such as 5.38 mg/kg(1/4 LD50),2.69 mg/kg (1/8 LD50) and control group (oil). The drug was given for 30 days by gavage. The rats were observed daily and weighted per week. At the end of experiment,the histopathological examinations of selected organs were done and the serum MDA,GSH-Px and SOD activity were measured. Results The treatment of abamectin significantly affected the body weight increment during experimental periods. There were statistical differences in the ratios of organs (brain, liver and kidney) to body weight among three groups of female rats (F=22.34、93.15、60.77,P〈0.05),and these ratios were significantly high in the treated female rats with 5.38 mg/kg. There were statistical differences in the serum MDA,GSH-Px and SOD activity among three groups (F = 57.48、120.62、30.45, P 〈 0.05),and they were significantly decreased in the treated group. The histopathological examination showed that the liver in the treated rats had obvious damages. Conclusion Subacute oral exposure to abamectin at 5.38 mg/kg could cause obvious toxicity,the liver might be sensitive organ and oxidative damage maybe play important role.
出处
《职业卫生与应急救援》
2014年第3期133-136,共4页
Occupational Health and Emergency Rescue
基金
黑龙江省自然基金项目(H201303)
黑龙江省卫生厅项目(2013121)